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PXD049081

PXD049081 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProximity labelling of mitochondria and endosomes upon mtDNA replication stress.
DescriptionThis project aims to identify novel regulators of mtDNA turnover, for what we used TurboID. For specific detection of the mitochondria-endosome proteome, we fused the N-terminal part of Turbo ID to RAB5C (TurboID aa1-aa72; RAB5C-SplitTurboNt-V5) and, the C-terminal part to SAMM50 (TurboID aa73–aa246; SAMM50-SplitTurboCt-HA). Proximity biotinylation was performed in transduced HEK293 cells expressing Split TurboID constructs. Cells expressing only SAMM50-SplitC-HA were used as a negative control. For protein isolation, HEK293 cells were incubated for 4 h with 0,25 mM biotin. For inducing mtDNA damage, cells were previously transfected with Twinkle K319E-Cherry 24 h before the biotin incubation.
HostingRepositoryPRIDE
AnnounceDate2025-04-07
AnnouncementXMLSubmission_2025-04-07_03:54:10.249.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPrerana Wagle
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-01 08:29:49ID requested
12025-04-07 03:54:10announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: mtDNA,Mitochondria, TurboID
Contact List
David Pla Martín
contact affiliation1. Institute of Physiology, University Clinics and Faculty of Medicine. University of Cologne, Germany 2. Center for Molecular Medicine (CMMC), University of Cologne. Germany 3. Institute of Biochemistry and Molecular Biology, University Clinics and Faculty of Medicine, Heinrich-Heine University Düsseldorf, Germany
contact emailplamartin@hhu.de
lab head
Prerana Wagle
contact affiliationCECAD Research Center
contact emailproteomics-facility@uni-koeln.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
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