PXD049054 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Light-induced targeting enables proteomics on endogenous condensates |
Description | Endogenous condensates with transient constituents are notoriously difficult to study with common biological assays like mass-spectrometry and other proteomics profiling. Here we report a method for light-induced targeting of endogenous condensates (LiTEC) in living cells. LiTEC combines the identification of molecular zip codes that target the endogenous condensates with optogenetics to enable controlled and reversible partitioning of an arbitrary cargo, such as enzymes commonly used in proteomics, into the condensate in a blue light dependent manner. We demonstrate a proof of concept by combining LiTEC with proximity-based biotinylation (BioID) and uncover putative components of transcriptional condensates in mouse embryonic stem cells. Our approach opens the road to genome-wide functional studies of endogenous condensates. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-25 |
AnnouncementXML | Submission_2024-10-25_03:31:29.077.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gerhard Mittler |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-31 13:58:45 | ID requested | |
⏵ 1 | 2024-10-25 03:31:29 | announced | |
Publication List
Keyword List
submitter keyword: pTEFb, RNA polymerase II transcription regulation, optoproteomics, TurboID,optogenetics, LFQ, biotin proximity labeling, BioID, Exploris480, phase separation |
Contact List
Gerhard Mittler |
contact affiliation | Max Planck Institute of Immunobiology and Epigenetics Proteomics Unit Stuebeweg 51 79108 Freiburg, Germany |
contact email | mittler@ie-freiburg.mpg.de |
lab head | |
Gerhard Mittler |
contact affiliation | Proteomics Unit, Max Planck Institute of Immunobiology and Epigenetics |
contact email | mittler@ie-freiburg.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD049054
- Label: PRIDE project
- Name: Light-induced targeting enables proteomics on endogenous condensates