PXD049054

PXD049054 is an original dataset announced via ProteomeXchange.

Title	Light-induced targeting enables proteomics on endogenous condensates
Description	Endogenous condensates with transient constituents are notoriously difficult to study with common biological assays like mass-spectrometry and other proteomics profiling. Here we report a method for light-induced targeting of endogenous condensates (LiTEC) in living cells. LiTEC combines the identification of molecular zip codes that target the endogenous condensates with optogenetics to enable controlled and reversible partitioning of an arbitrary cargo, such as enzymes commonly used in proteomics, into the condensate in a blue light dependent manner. We demonstrate a proof of concept by combining LiTEC with proximity-based biotinylation (BioID) and uncover putative components of transcriptional condensates in mouse embryonic stem cells. Our approach opens the road to genome-wide functional studies of endogenous condensates.
HostingRepository	PRIDE
AnnounceDate	2024-10-25
AnnouncementXML	Submission_2024-10-25_03:31:29.077.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerhard Mittler
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-31 13:58:45	ID requested
⏵ 1	2024-10-25 03:31:29	announced

10.1016/J.CELL.2024.09.040;

submitter keyword: pTEFb, RNA polymerase II transcription regulation, optoproteomics, TurboID,optogenetics, LFQ, biotin proximity labeling, BioID, Exploris480, phase separation

Gerhard Mittler
contact affiliation	Max Planck Institute of Immunobiology and Epigenetics Proteomics Unit Stuebeweg 51 79108 Freiburg, Germany
contact email	mittler@ie-freiburg.mpg.de
lab head
Gerhard Mittler
contact affiliation	Proteomics Unit, Max Planck Institute of Immunobiology and Epigenetics
contact email	mittler@ie-freiburg.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD049054

PRIDE project URI

• PRIDE
  1. PXD049054
     1. Label: PRIDE project
     2. Name: Light-induced targeting enables proteomics on endogenous condensates