PXD049031

PXD049031 is an original dataset announced via ProteomeXchange.

Title	A new cellular platform for studying autophagy
Description	Atg8 proteins play a crucial role in autophagy. In yeast, there is a single Atg8 member, while mammals have six homologs categorized into LC3s and GABARAPs. The GABARAP subfamily consists of GABARAP, GABARAPL1, and GABARAPL2/GATE16 proteins, contributing to various autophagy stages. However, the intricacies among GABARAP proteins are complex and not well-understood. We introduce a new cellular platform to study autophagy, achieved by tagging GABARAP genes with N’-terminal 3xFlag epitopes fused to Clover or mScarletI fluorescent proteins using CRISPR/Cas9. This platform offers robust autophagy examination via flow cytometry, enabling real-time monitoring of GABARAP homologs at single-cell resolution through fluorescent microscopy. The 3xFlag epitope facilitates isolation and characterization of distinct autophagosomes labeled by specific GABARAP homologs. Our platform promises insights into the unique roles of GABARAP homologs in autophagy and possibly other cellular processes.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:06:17.077.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yishai Levin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-31 01:17:23	ID requested
⏵ 1	2025-05-06 13:06:17	announced

10.1016/j.jbc.2024.107908;

Goldin-Azulay K, Fraiberg M, Trofimyuk O, Levin Y, Reuven N, Kopitman E, Elazar Z, Multiplex genomic tagging of mammalian ATG8s to study autophagy. J Biol Chem, 300(12):107908(2024) [pubmed]

submitter keyword: proteomics, GABARAP, immunoprecipitation,Autophagy

Zvulun Elazar
contact affiliation	Department of Biomolecular Sciences The Weizmann Institute of Science
contact email	Zvulun.Elazar@weizmann.ac.il
lab head
Yishai Levin
contact affiliation	Weizmann Institute of Science, Israel
contact email	yishai.levin@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD049031

PRIDE project URI

• PRIDE
  1. PXD049031
     1. Label: PRIDE project
     2. Name: A new cellular platform for studying autophagy