PXD049026

PXD049026 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic identification of the hepatopancreas response to VpAHPND-OMVs in penaeus vannamei
Description	Experimental group 1 and experimental group 2 were fed with shrimp feed containing 30 μ g/kg and 60 μ g/kg OMVs respectively, while the control group was not given any basic diet without any treatment. The feeding experiment of Penaeus vannamei was carried out in the indoor culture workshop of Guangdong Lanyin Aquatic Science and Technology Company in Wenchang City, Hainan Province. After the end of the domestication period, healthy Litopenaeus vannamei were randomly divided into 3 groups with 3 repeats in each group and 40 prawns in each group. The daily feeding amount is 5% of shrimp body weight and 8% of shrimp body weight, and the feeding amount is adjusted at any time according to the feeding situation of shrimp fry. Feed three times a day at 7:00, 14:00 and 20:00 every day, respectively. In order to keep the water environment of cultured shrimp stable, it was ventilated continuously for 24 hours during the experiment, and the temperature, salinity, pH value and ammonia nitrogen concentration were basically the same. Replace the original water body with new sand filtered seawater every 2 days, and remove unconsumed feed and fecal residues from each tank 2 hours after eating. Pay attention to the health status of prawns during the experiment. On the 28th day of the experimental cycle, 9 shrimps were randomly taken from each group, and the hepatopancreas were dissected and frozen in liquid nitrogen for 15 min, then immediately transferred to the refrigerator at -80 ℃ for preservation. Proteomic sequencing to probe the biological role of VpAHPND-OMVs.
HostingRepository	iProX
AnnounceDate	2024-01-31
AnnouncementXML	Submission_2024-05-05_21:14:46.619.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lihan Wang
SpeciesList	scientific name: Penaeus vannamei; NCBI TaxID: 6689;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-30 20:04:49	ID requested
⏵ 1	2024-05-05 21:14:47	announced

Dataset with its publication pending

submitter keyword: Comparative proteomic, penaeus vannamei, VpAHPND-OMVs, Innate immunity

Mengqiang Wang
contact affiliation	MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China
contact email	wangmengqiang@ouc.edu.cn
lab head
Lihan Wang
contact affiliation	MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China
contact email	2648181506@qq.com
dataset submitter

iProX dataset URI