PXD049021

PXD049021 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Col-0_srk2de_abi1-1C_GCPs_Proteomics
Description	Gas exchange between plants and the atmosphere is critical for regulating plant growth or stress responses. When plants are exposed to drought stress, abscisic acid (ABA)-activated SnRK2 protein kinases play a central role for stomatal closure to prevent excessive water loss. To identify SnRK2 substrates in guard cells, a cell-type-specific phosphoproteomic analysis was performed, and MAP4K1 and MAP4K2 were identified as putative signaling factors in guard cells. MAP4K1 and MAP4K2 function redundantly in guard cells, as a map4k1map4k2 mutant impaired ABA-induced stomatal closure. MAP4K1 Ser-479 is directly phosphorylated by SRK2E (OST1/SnRK2.6), and it physiologically interacts with SRK2E in planta, suggesting that MAP4K1 is in vivo substrates of SnRK2. Mutational analysis revealed that phosphorylation of MAP4K1 Ser-479 is required for ABA-induced stomatal closure. Furthermore, an electrophysiological assay suggested that MAP4K1/2 are involved in the ABA-responsive activation of Ca2+-permeable channels. Collectively, our results propose that ABA-activated SnRK2 and MAP4K1/2 constitute a signaling pathway that positively regulates ABA-induced stomatal closure via Ca2+ signaling.
HostingRepository	jPOST
AnnounceDate	2025-01-30
AnnouncementXML	Submission_2025-01-29_07:01:12.521.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Taishi Umezawa
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-30 17:50:29	ID requested
⏵ 1	2025-01-29 07:01:12	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Arabidopsis, Guard cell, Proteome, Abscisic acid

submitter keyword: Arabidopsis, Guard cell, Proteome, Abscisic acid

Contact List

Kota Yamashita
lab head
Taishi Umezawa
contact affiliation	Tokyo University of Agriculture and Technology
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002486/