PXD049019

PXD049019 is an original dataset announced via ProteomeXchange.

Title	Small extracellular vesicles promote stiffness-mediated metastasis
Description	Tissue stiffness is a critical prognostic factor in breast cancer and is associated with metastatic progression. Here we show an alternative and complementary hypothesis of tumor progression whereby physiological matrix stiffness affects the quantity and protein cargo of small EVs produced by cancer cells, which in turn aid cancer cell dissemination. Primary patient breast tissue produces significantly more EVs from stiff tumor tissue than soft tumor adjacent tissue. EVs released by cancer cells on matrices that model human breast tumors (25 kPa; stiff EVs) feature increased adhesion molecule presentation (ITGα2β1, ITGα6β4, ITGα6β1, CD44) compared to EVs from softer normal tissue (0.5 kPa; soft EVs), which facilitates their binding to extracellular matrix (ECM) protein collagen IV, and a 3-fold increase in homing ability to distant organs in mice. In a zebrafish xenograft model, stiff EVs aid cancer cell dissemination. Moreover, normal, resident lung fibroblasts treated with stiff and soft EVs change their gene expression profiles to adopt a cancer associated fibroblast (CAF) phenotype. These findings show that EV quantity, cargo, and function depend heavily on the mechanical properties of the extracellular microenvironment.
HostingRepository	PRIDE
AnnounceDate	2024-06-22
AnnouncementXML	Submission_2024-06-22_08:30:50.009.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD049019
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bart Starich
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-30 15:38:36	ID requested
⏵ 1	2024-06-22 08:30:51	announced

10.1101/2023.07.01.545937;

10.1158/2767-9764.crc-23-0431;

10.6019/PXD049019;

Sneider A, Liu Y, Starich B, Du W, Marar C, Faqih N, Ciotti GE, Kim JH, Krishnan S, Ibrahim S, Igboko M, Locke A, Lewis DM, Hong H, Karl M, Vij R, Russo GC, Nair P, G, ó, mez-de-Mariscal E, Habibi M, Mu, ñ, oz-Barrutia A, Gu L, Eisinger-Mathason TSK, Wirtz D, Small extracellular vesicles promote stiffness-mediated metastasis. bioRxiv, ():(2024) [pubmed]

Sneider A, Liu Y, Starich B, Du W, Nair PR, Marar C, Faqih N, Ciotti GE, Kim JH, Krishnan S, Ibrahim S, Igboko M, Locke A, Lewis DM, Hong H, Karl MN, Vij R, Russo GC, G, ó, mez-de-Mariscal E, Habibi M, Mu, ñ, oz-Barrutia A, Gu L, Eisinger-Mathason TSK, Wirtz D, Small Extracellular Vesicles Promote Stiffness-mediated Metastasis. Cancer Res Commun, 4(5):1240-1252(2024) [pubmed]

submitter keyword: Human, Breast Cancer, Extracellular Vesicles, Tissue Stiffness

Denis Wirtz
contact affiliation	Chemical & Biomolecular Engineering, Johns Hopkins University
contact email	wirtz@jhu.edu
lab head
Bart Starich
contact affiliation	Johns Hopkins University, Institute for Nanobiotechnology
contact email	bstaric1@jhu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD049019

PRIDE project URI

• PRIDE
  1. PXD049019
     1. Label: PRIDE project
     2. Name: Small extracellular vesicles promote stiffness-mediated metastasis