PXD049019
PXD049019 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Small extracellular vesicles promote stiffness-mediated metastasis |
Description | Tissue stiffness is a critical prognostic factor in breast cancer and is associated with metastatic progression. Here we show an alternative and complementary hypothesis of tumor progression whereby physiological matrix stiffness affects the quantity and protein cargo of small EVs produced by cancer cells, which in turn aid cancer cell dissemination. Primary patient breast tissue produces significantly more EVs from stiff tumor tissue than soft tumor adjacent tissue. EVs released by cancer cells on matrices that model human breast tumors (25 kPa; stiff EVs) feature increased adhesion molecule presentation (ITGα2β1, ITGα6β4, ITGα6β1, CD44) compared to EVs from softer normal tissue (0.5 kPa; soft EVs), which facilitates their binding to extracellular matrix (ECM) protein collagen IV, and a 3-fold increase in homing ability to distant organs in mice. In a zebrafish xenograft model, stiff EVs aid cancer cell dissemination. Moreover, normal, resident lung fibroblasts treated with stiff and soft EVs change their gene expression profiles to adopt a cancer associated fibroblast (CAF) phenotype. These findings show that EV quantity, cargo, and function depend heavily on the mechanical properties of the extracellular microenvironment. |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-22 |
AnnouncementXML | Submission_2024-06-22_08:30:50.009.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD049019 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Bart Starich |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-01-30 15:38:36 | ID requested | |
⏵ 1 | 2024-06-22 08:30:51 | announced |
Publication List
10.1101/2023.07.01.545937; |
10.1158/2767-9764.crc-23-0431; |
10.6019/PXD049019; |
Sneider A, Liu Y, Starich B, Du W, Marar C, Faqih N, Ciotti GE, Kim JH, Krishnan S, Ibrahim S, Igboko M, Locke A, Lewis DM, Hong H, Karl M, Vij R, Russo GC, Nair P, G, ó, mez-de-Mariscal E, Habibi M, Mu, ñ, oz-Barrutia A, Gu L, Eisinger-Mathason TSK, Wirtz D, Small extracellular vesicles promote stiffness-mediated metastasis. bioRxiv, ():(2024) [pubmed] |
Sneider A, Liu Y, Starich B, Du W, Nair PR, Marar C, Faqih N, Ciotti GE, Kim JH, Krishnan S, Ibrahim S, Igboko M, Locke A, Lewis DM, Hong H, Karl MN, Vij R, Russo GC, G, ó, mez-de-Mariscal E, Habibi M, Mu, ñ, oz-Barrutia A, Gu L, Eisinger-Mathason TSK, Wirtz D, Small Extracellular Vesicles Promote Stiffness-mediated Metastasis. Cancer Res Commun, 4(5):1240-1252(2024) [pubmed] |
Keyword List
submitter keyword: Human, Breast Cancer, Extracellular Vesicles, Tissue Stiffness |
Contact List
Denis Wirtz | |
---|---|
contact affiliation | Chemical & Biomolecular Engineering, Johns Hopkins University |
contact email | wirtz@jhu.edu |
lab head | |
Bart Starich | |
contact affiliation | Johns Hopkins University, Institute for Nanobiotechnology |
contact email | bstaric1@jhu.edu |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD049019 |
PRIDE project URI |
Repository Record List
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