PXD049002

PXD049002 is an original dataset announced via ProteomeXchange.

Title	Fracture hematoma proteomics: surgical invasiveness characterizes the early fracture healing cascade after multiple trauma
Description	Purpose The aim of this study was to determine the fracture hematoma (fxH) proteome after multiple trauma using label free proteomics, comparing two different established surgical treatment strategies. Basic procedures A large animal (sus scrofa), multiple trauma animal model was used in which two different trauma surgical treatment methods were compared: Early Total Care (ETC) versus Damage Control Orthopedics (DCO). fxH was harvested after 72 hours and analyzed using liquid chromatography-tandem mass spectrometry. Protein interaction analyses were performed to further elucidate key biomolecular pathways in the early fracture healing phase. Main findings The proteome of the early fxH was characterized by immunomodulatory and osteogenic proteins, as well as proteins involved in the coagulation cascade. Distinct, treatment-specific proteome alterations were observed. The fxH proteome of the ETC group showed increased expression of pro-inflammatory proteins related to, among others, activation of the complement system, neutrophil functioning, and macrophage activation, while showing decreased expression of proteins related to osteogenesis and tissue remodeling. On the other hand, the fxH proteome of the DCO group contained various upregulated or exclusively detected proteins related to tissue regeneration and remodeling, as well as proteins related to anti-inflammatory and osteogenic processes. Principal conclusions The early fxH proteome of the ETC group was characterized by the expression of immunomodulatory, mainly pro-inflammatory, proteins, whereas the early fxH proteome of the DCO group was more regenerative and osteogenic in nature. These findings match clinical observations, in which enhanced surgical trauma after severe multiple trauma causes dysbalanced inflammation, potentially leading to reduced tissue regenerative capabilities, and gained insights into regulatory mechanisms of fracture healing after severe trauma.
HostingRepository	PRIDE
AnnounceDate	2024-05-24
AnnouncementXML	Submission_2024-05-24_03:55:06.473.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD049002
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christel Kuik
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-30 04:35:42	ID requested
⏵ 1	2024-05-24 03:55:07	announced

10.1302/2046-3758.135.bjr-2023-0323.r1;

Groven RVM, Kuik C, Greven J, Mert Ü, Bouwman FG, Poeze M, Blokhuis TJ, Huber-Lang M, Hildebrand F, Cillero-Pastor B, van Griensven M, Fracture haematoma proteomics. Bone Joint Res, 13(5):214-225(2024) [pubmed]

10.6019/PXD049002;

submitter keyword: proteomics, Trauma, LC-MS, Fracture Hematoma

Martijn van Griensven
contact affiliation	Department of Cell Biology-Inspired Tissue Engineering, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, The Netherlands.
contact email	m.vangriensven@maastrichtuniversity.nl
lab head
Christel Kuik
contact affiliation	Maastricht MultiModal Molecular imaging institute (M4i), Maastricht University, Maastricht, the netherlands
contact email	christel.kuik@maastrichtuniversity.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049002
     1. Label: PRIDE project
     2. Name: Fracture hematoma proteomics: surgical invasiveness characterizes the early fracture healing cascade after multiple trauma