PXD048973

PXD048973 is an original dataset announced via ProteomeXchange.

Title	Integrative analysis of the native ethanolamine utilization bacterial microcompartment system in Escherichia coli.
Description	Bacterial microcompartments (BMCs) represent prokaryotic counterparts to the eukaryotic organelles. BMCs act as intracellular microbioreactors that spatially insulate targeted pathways and enhance their kinetics through metabolic channelling effects. Some strains of E. coli natively bear an ethanolamine utilization (eut) operon with all the genetic elements required to produce Eut BMCs. The E. coli Eut BMCs could represent powerful tools for synthetic biology applications, such as encapsulating heterologous pathways in vivo to improve their yields. However, they remain poorly characterized. We have thus implemented a systems biology approach to evaluate whether E. coli can produce well-assembled and functional Eut BMCs, focusing on the laboratory strain E. coli K12 W3110. Using 13C-tracer fluxomics data and modelling based approaches, we first characterized the ethanolamine metabolism. We then observed the Eut BMCs by transmission electron microscopy as well as cryotomography. Moreover, we generated novel insights into the Eut BMCs composition through a proteomics analysis. Altogether, these results allowed us to understand how the E. coli Eut BMC operates and how to hack its function while maintaining its integrity.
HostingRepository	PRIDE
AnnounceDate	2024-07-02
AnnouncementXML	Submission_2024-07-02_02:32:13.317.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandre STELLA
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-29 07:02:15	ID requested
⏵ 1	2024-07-02 02:32:13	announced

10.1128/MSYSTEMS.00750-24;

submitter keyword: Escherichia, bacterial microcompartment, ethanolamine

Odile Schiltz
contact affiliation	Proteomics and Mass Spectrometry of Biomolecules, ProteoToul Research Proteomics Facility of Toulouse, ProteoToul Services Proteomics French Infrastructure, ProFI IPBS, CNRS, UMR 5089 205 route de Narbonne BP 64182 31077 Toulouse Cedex France
contact email	Odile.Schiltz@ipbs.fr
lab head
Alexandre STELLA
contact affiliation	CNRS
contact email	alexandre.stella@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD048973

PRIDE project URI

• PRIDE
  1. PXD048973
     1. Label: PRIDE project
     2. Name: Integrative analysis of the native ethanolamine utilization bacterial microcompartment system in Escherichia coli.