PXD048973 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Integrative analysis of the native ethanolamine utilization bacterial microcompartment system in Escherichia coli. |
Description | Bacterial microcompartments (BMCs) represent prokaryotic counterparts to the eukaryotic organelles. BMCs act as intracellular microbioreactors that spatially insulate targeted pathways and enhance their kinetics through metabolic channelling effects. Some strains of E. coli natively bear an ethanolamine utilization (eut) operon with all the genetic elements required to produce Eut BMCs. The E. coli Eut BMCs could represent powerful tools for synthetic biology applications, such as encapsulating heterologous pathways in vivo to improve their yields. However, they remain poorly characterized. We have thus implemented a systems biology approach to evaluate whether E. coli can produce well-assembled and functional Eut BMCs, focusing on the laboratory strain E. coli K12 W3110. Using 13C-tracer fluxomics data and modelling based approaches, we first characterized the ethanolamine metabolism. We then observed the Eut BMCs by transmission electron microscopy as well as cryotomography. Moreover, we generated novel insights into the Eut BMCs composition through a proteomics analysis. Altogether, these results allowed us to understand how the E. coli Eut BMC operates and how to hack its function while maintaining its integrity. |
HostingRepository | PRIDE |
AnnounceDate | 2024-07-02 |
AnnouncementXML | Submission_2024-07-02_02:32:13.317.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alexandre STELLA |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-29 07:02:15 | ID requested | |
⏵ 1 | 2024-07-02 02:32:13 | announced | |
Publication List
Keyword List
submitter keyword: Escherichia, bacterial microcompartment, ethanolamine |
Contact List
Odile Schiltz |
contact affiliation | Proteomics and Mass Spectrometry of Biomolecules, ProteoToul Research Proteomics Facility of Toulouse, ProteoToul Services Proteomics French Infrastructure, ProFI IPBS, CNRS, UMR 5089 205 route de Narbonne BP 64182 31077 Toulouse Cedex France |
contact email | Odile.Schiltz@ipbs.fr |
lab head | |
Alexandre STELLA |
contact affiliation | CNRS |
contact email | alexandre.stella@ipbs.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048973
- Label: PRIDE project
- Name: Integrative analysis of the native ethanolamine utilization bacterial microcompartment system in Escherichia coli.