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PXD048973

PXD048973 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntegrative analysis of the native ethanolamine utilization bacterial microcompartment system in Escherichia coli.
DescriptionBacterial microcompartments (BMCs) represent prokaryotic counterparts to the eukaryotic organelles. BMCs act as intracellular microbioreactors that spatially insulate targeted pathways and enhance their kinetics through metabolic channelling effects. Some strains of E. coli natively bear an ethanolamine utilization (eut) operon with all the genetic elements required to produce Eut BMCs. The E. coli Eut BMCs could represent powerful tools for synthetic biology applications, such as encapsulating heterologous pathways in vivo to improve their yields. However, they remain poorly characterized. We have thus implemented a systems biology approach to evaluate whether E. coli can produce well-assembled and functional Eut BMCs, focusing on the laboratory strain E. coli K12 W3110. Using 13C-tracer fluxomics data and modelling based approaches, we first characterized the ethanolamine metabolism. We then observed the Eut BMCs by transmission electron microscopy as well as cryotomography. Moreover, we generated novel insights into the Eut BMCs composition through a proteomics analysis. Altogether, these results allowed us to understand how the E. coli Eut BMC operates and how to hack its function while maintaining its integrity.
HostingRepositoryPRIDE
AnnounceDate2024-07-02
AnnouncementXMLSubmission_2024-07-02_02:32:13.317.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlexandre STELLA
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-29 07:02:15ID requested
12024-07-02 02:32:13announced
Publication List
10.1128/MSYSTEMS.00750-24;
Keyword List
submitter keyword: Escherichia, bacterial microcompartment, ethanolamine
Contact List
Odile Schiltz
contact affiliationProteomics and Mass Spectrometry of Biomolecules, ProteoToul Research Proteomics Facility of Toulouse, ProteoToul Services Proteomics French Infrastructure, ProFI IPBS, CNRS, UMR 5089 205 route de Narbonne BP 64182 31077 Toulouse Cedex France
contact emailOdile.Schiltz@ipbs.fr
lab head
Alexandre STELLA
contact affiliationCNRS
contact emailalexandre.stella@ipbs.fr
dataset submitter
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Dataset FTP location
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