PXD048952

PXD048952 is an original dataset announced via ProteomeXchange.

Title	Targeting REGγ proteasome to facilitate target protein degradation
Description	As a member of the 11S proteasome family, REGγ facilitates ubiquitin-independent protein degradation. Previous studies have largely concentrated on the effects of substrate protein degradation. However, the mechanism by which REGγ recognizes substrate proteins and the substrate selectivity of REGγ remained unclear. In this study, we discovered that the multiple substrate proteins of REGγ contain the RKRR conserved sequence. Subsequently, the degron of REGγ, RR(K)R(K)R, was ascertained through site mutation, BLI, and Cryo-EM analysis. Consequently, the recognition mechanism of REGγ and its substrate proteins was clarified. Simultaneously, the number of substrates was expanded through a combination of interactome and degron analysis. Moreover, we successfully constructed a targeted protein degradation system that utilizes the degron of REGγ, thereby providing a novel approach for targeted protein degradation.
HostingRepository	iProX
AnnounceDate	2024-01-27
AnnouncementXML	Submission_2024-04-22_18:50:46.466.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bo Yang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-28 20:09:14	ID requested
⏵ 1	2024-04-22 18:50:46	announced

Dataset with its publication pending

submitter keyword: REGγ, target protein degradation

Lei Li
contact affiliation	East China Normal University
contact email	lli@bio.ecnu.edu.cn
lab head
Bo Yang
contact affiliation	East China Normal University
contact email	yangbo6674@163.com
dataset submitter

iProX dataset URI