PXD048935

PXD048935 is an original dataset announced via ProteomeXchange.

Title	Mcm7 ubiquitylation and Pif1-family helicases drive CMG disassembly to preserve genome integrity
Description	Disassembly of the DNA helicase known as CMG (Cdc45-Mcm-GINS) is the key regulated step during DNA replication termination in eukaryotes. In budding yeast, CMG disassembly is initiated by the ubiquitin ligase SCF-Dia2, which ubiquitylates the Mcm7 subunit of CMG, leading to recruitment of the Cdc48 unfoldase. Here we have investigated the partners of the F-box protein Dia2 and tested which ones are dependent upon the TPR domain of Dia2, using yeast strains expressing ProteinA-tagged Dia2 or Dia2∆TPR. Controls include a strain expressing the TAP tag alone, and a strain expressing TAP-tagged Sld5 subunit of the CMG helicase.
HostingRepository	PRIDE
AnnounceDate	2025-04-09
AnnouncementXML	Submission_2025-04-09_04:20:29.769.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD048935
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	KARIM LABIB
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-27 08:46:38	ID requested
⏵ 1	2025-04-09 04:20:30	announced

10.1038/S44318-024-00161-X;

10.6019/PXD048935;

submitter keyword: ubiquitylation, Dia2, CMG helicase, CMG disassembly, Pif1,S. cerevisiae, DNA replication, Rrm3

Professor Karim Labib
contact affiliation	MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, DD1 5EH, U.K.
contact email	k.p.m.labib@dundee.ac.uk
lab head
KARIM LABIB
contact affiliation	University of Dundee
contact email	kpmlabib@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048935
     1. Label: PRIDE project
     2. Name: Mcm7 ubiquitylation and Pif1-family helicases drive CMG disassembly to preserve genome integrity