PXD048930

PXD048930 is an original dataset announced via ProteomeXchange.

Title	Analysis of N-Glycosylation for SARS-CoV-2 expressed receptor-binding domain VOCs and membrane protein with LC-MS.
Description	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can infect human cells by first attaching to the ACE-2 receptor via its receptor-binding domain (RBD) in the spike protein. We investigated the influence of N-glycosylation sites of the RBD and the membrane (M) protein on IgG antibody binding in serum samples from patients infected with the original SARS-CoV-2 strain in Germany. The N-glycosylation sites Asn331, Asn334, Asn343, Asn370 and Asn381 of RBDs of the wildtype, alpha, beta, gamma, kappa, and omicron BA.1 variants expressed in HEK293T and HEK293S GnTI- cells , as well as Asn5 for the M-protein were analyzed with tandem mass spectrometry for N-glycosylation.
HostingRepository	PanoramaPublic
AnnounceDate	2024-04-29
AnnouncementXML	Submission_2024-04-29_14:21:06.594.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Daniela Volke
SpeciesList	scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049;
ModificationList	Deamidated; Hex(5)HexNAc(2); Carbamidomethyl; HexNAc; Oxidation
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-26 17:24:25	ID requested
1	2024-03-26 22:51:50	announced
⏵ 2	2024-04-29 14:21:07	announced	: Added PubMed Id

Schwarze M, Volke D, Rojas Echeverri JC, Schick R, Lakowa N, Gr, ü, newald T, Wolf J, Borte S, Scholz M, Krizsan A, Hoffmann R, Influence of Mutations and N-Glycosylation Sites in the Receptor-Binding Domain (RBD) and the Membrane Protein of SARS-CoV-2 Variants of Concern on Antibody Binding in ELISA. Biology (Basel), 13(4):(2024) [pubmed]

submitter keyword: SARS-CoV-2, N-Glycosylation, HD-DDA, ESI-IMS-Q-TOF-MS

Ralf Hoffmann
contact affiliation	Universität Leipzig/BBZ
contact email	ralf.hoffmann@bbz.uni-leipzig.de
lab head
Daniela Volke
contact affiliation	Universität Leipzig/BBZ
contact email	daniela.volke@bbz.uni-leipzig.de
dataset submitter

Panorama Public dataset URI