PXD048930

PXD048930 is an original dataset announced via ProteomeXchange.

Title	Analysis of N-Glycosylation for SARS-CoV-2 expressed receptor-binding domain VOCs and membrane protein with LC-MS.
Description	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can infect human cells by first attaching to the ACE-2 receptor via its receptor-binding domain (RBD) in the spike protein. We investigated the influence of N-glycosylation sites of the RBD and the membrane (M) protein on IgG antibody binding in serum samples from patients infected with the original SARS-CoV-2 strain in Germany. The N-glycosylation sites Asn331, Asn334, Asn343, Asn370 and Asn381 of RBDs of the wildtype, alpha, beta, gamma, kappa, and omicron BA.1 variants expressed in HEK293T and HEK293S GnTI- cells , as well as Asn5 for the M-protein were analyzed with tandem mass spectrometry for N-glycosylation.
HostingRepository	PanoramaPublic
AnnounceDate	2024-03-26
AnnouncementXML	Submission_2024-03-26_22:51:50.185.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Daniela Volke
SpeciesList	scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049;
ModificationList	Deamidated; Hex(5)HexNAc(2); Carbamidomethyl; HexNAc; Oxidation
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-26 17:24:25	ID requested
⏵ 1	2024-03-26 22:51:50	announced

Schwarze M, Volke D, Rojas Echeverri JC, Schick R, Lakowa N, Grünewald T, Wolf J, Borte S, Scholz M, Krizsan A, et al. Influence of Mutations and N-Glycosylation Sites in the Receptor-Binding Domain (RBD) and the Membrane Protein of SARS-CoV-2 Variants of Concern on Antibody Binding in ELISA. Biology. 2024

13(4):207. https://doi.org/10.3390/biology13040207

submitter keyword: SARS-CoV-2, N-Glycosylation, HD-DDA, ESI-IMS-Q-TOF-MS

Ralf Hoffmann
contact affiliation	Universität Leipzig/BBZ
contact email	ralf.hoffmann@bbz.uni-leipzig.de
lab head
Daniela Volke
contact affiliation	Universität Leipzig/BBZ
contact email	daniela.volke@bbz.uni-leipzig.de
dataset submitter

Panorama Public dataset URI