PXD048928

PXD048928 is an original dataset announced via ProteomeXchange.

Title	Proteomic diversification of spermatostyles among six species of whirligig beetles
Description	Seminal fluid protein (SFP) composition is complex and commonly assumed to be rapidly divergent due to functional interactions with both sperm and the female reproductive tract (FRT), both of which evolve rapidly. In addition to sperm, seminal fluid may contain structures, such as mating plugs and spermatophores. Here, we investigate the evolutionary diversification of a lesser-known ejaculate structure: the spermatostyle, which has independently arisen in several families of beetles and true bugs. We characterized the spermatostyle proteome, in addition to spermatostyle and FRT morphology, in six species of whirligig beetles (family Gyrinidae). Spermatostyles were enriched for proteolytic enzymes, and assays confirmed they possess proteolytic activity. Sperm leucyl aminopeptidases (S-LAPs) were particularly abundant, and their localization to spermatostyles was confirmed by immunohistochemistry. Although there was evidence for functional conservation of spermatostyle proteomes across species, phylogenetic regressions suggest evolutionary covariation between protein composition and the morphology of both spermatostyles and FRTs. We postulate that S-LAPs (and other proteases) have evolved a novel structural role in spermatostyles and discuss spermatostyles as adaptations for delivering male-derived materials to females.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:53:41.183.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD048928
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Roberto Gomez
SpeciesList	scientific name: Dineutus ciliatus; NCBI TaxID: 1241446; scientific name: Orectochilus villosus; NCBI TaxID: 107782; scientific name: Dineutus hornii; NCBI TaxID: 1564125; scientific name: Dineutus discolor; NCBI TaxID: 1241447; scientific name: Dineutus assimilis; NCBI TaxID: 1720665; scientific name: Dineutus nigrior; NCBI TaxID: 1564128;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-26 14:12:11	ID requested
1	2024-08-09 03:22:48	announced
⏵ 2	2024-10-22 06:53:49	announced	2024-10-22: Updated project metadata.

10.1002/mrd.23745;

10.6019/PXD048928;

Gomez RA, Dallai R, Sims-West DJ, Mercati D, Sinka R, Ahmed-Braimah Y, Pitnick S, Dorus S, Proteomic diversification of spermatostyles among six species of whirligig beetles. Mol Reprod Dev, 91(5):e23745(2024) [pubmed]

submitter keyword: Reproduction, Fertility, Sexual selection,Evolution, Coleoptera

Steve Dorus
contact affiliation	Center for Reproductive Evolution, Department of Biology, Syracuse University, United States of America
contact email	sdorus@syr.edu
lab head
Roberto Gomez
contact affiliation	Syracuse University
contact email	rgomez02@syr.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048928
     1. Label: PRIDE project
     2. Name: Proteomic diversification of spermatostyles among six species of whirligig beetles