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PXD048924

PXD048924 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLack of AtMC1 catalytic activity triggers autoimmunity dependent on NLR stability
DescriptionPlants utilize cell surface-localized pattern recognition receptors (PRRs) and intracellular nucleotide-binding leucine-rich repeat (NLR) receptors to detect non-self and elicit robust immune responses. During plant development and especially during aging, fine-tuning the homeostasis of these receptors is critical to prevent their hyperactivation. Here, we show that Arabidopsis plants lacking metacaspase 1 (AtMC1) display autoimmunity dependent on immune signalling components downstream of NLR and PRR activation. Overexpression of catalytically inactive AtMC1 in an atmc1 background triggers severe autoimmunity partially dependent on the same immune signalling components. Although individual mutations in NLRs, PRRs or other immune-related components that interact with catalytically inactive AtMC1 do not rescue the autoimmune phenotype, overexpression of SNIPER1, a master regulator of NLR homeostasis, fully attenuates the phenotype, inferring that a broad defect in NLR turnover may underlie the severe autoimmunity observed. As opposed to Wt AtMC1 which exhibits a nucleocytoplasmic localization, catalytically inactive AtMC1 localizes to puncta structures that are degraded through autophagy. We infer that the phenotypes observed in plants overexpressing catalytically inactive AtMC1 may represent an additive phenotype to the relatively milder autoimmunity observed in atmc1 mutants. Altogether and considering previous evidence on the proteostatic functions of AtMC1, we speculate that Wt AtMC1 may either directly or indirectly control NLR protein levels as plants approach adulthood, thus preventing autoimmunity.
HostingRepositoryPRIDE
AnnounceDate2025-03-21
AnnouncementXMLSubmission_2025-03-21_01:41:05.968.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEsperanza Fernandez
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-26 13:59:54ID requested
12025-03-21 01:41:06announced
Publication List
10.1038/S44319-025-00426-4;
Keyword List
submitter keyword: Autoimmunity, Proteostasis, Plant immunity, Autophagy, Metacaspases, Condensates, NLRs
Contact List
Nuria Sanchez Coll
contact affiliationCentre for Reseach in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, 08193, Spain Consejo Superior de Investigaciones Cientificas (CSIC), Barcelona, 08001, Spain
contact emailnuria.sanchez-coll@cragenomica.es
lab head
Esperanza Fernandez
contact affiliationVIB-UGent
contact emailesperanza.fernandez@vib-ugent.be
dataset submitter
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Dataset FTP location
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