PXD048924

PXD048924 is an original dataset announced via ProteomeXchange.

Title	Lack of AtMC1 catalytic activity triggers autoimmunity dependent on NLR stability
Description	Plants utilize cell surface-localized pattern recognition receptors (PRRs) and intracellular nucleotide-binding leucine-rich repeat (NLR) receptors to detect non-self and elicit robust immune responses. During plant development and especially during aging, fine-tuning the homeostasis of these receptors is critical to prevent their hyperactivation. Here, we show that Arabidopsis plants lacking metacaspase 1 (AtMC1) display autoimmunity dependent on immune signalling components downstream of NLR and PRR activation. Overexpression of catalytically inactive AtMC1 in an atmc1 background triggers severe autoimmunity partially dependent on the same immune signalling components. Although individual mutations in NLRs, PRRs or other immune-related components that interact with catalytically inactive AtMC1 do not rescue the autoimmune phenotype, overexpression of SNIPER1, a master regulator of NLR homeostasis, fully attenuates the phenotype, inferring that a broad defect in NLR turnover may underlie the severe autoimmunity observed. As opposed to Wt AtMC1 which exhibits a nucleocytoplasmic localization, catalytically inactive AtMC1 localizes to puncta structures that are degraded through autophagy. We infer that the phenotypes observed in plants overexpressing catalytically inactive AtMC1 may represent an additive phenotype to the relatively milder autoimmunity observed in atmc1 mutants. Altogether and considering previous evidence on the proteostatic functions of AtMC1, we speculate that Wt AtMC1 may either directly or indirectly control NLR protein levels as plants approach adulthood, thus preventing autoimmunity.
HostingRepository	PRIDE
AnnounceDate	2025-03-21
AnnouncementXML	Submission_2025-03-21_01:41:05.968.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Esperanza Fernandez
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-26 13:59:54	ID requested
⏵ 1	2025-03-21 01:41:06	announced

10.1038/S44319-025-00426-4;

submitter keyword: Autoimmunity, Proteostasis, Plant immunity, Autophagy, Metacaspases, Condensates, NLRs

Nuria Sanchez Coll
contact affiliation	Centre for Reseach in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, 08193, Spain Consejo Superior de Investigaciones Cientificas (CSIC), Barcelona, 08001, Spain
contact email	nuria.sanchez-coll@cragenomica.es
lab head
Esperanza Fernandez
contact affiliation	VIB-UGent
contact email	esperanza.fernandez@vib-ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048924
     1. Label: PRIDE project
     2. Name: Lack of AtMC1 catalytic activity triggers autoimmunity dependent on NLR stability