PXD048921
PXD048921 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The senescence-associated cell surfaceome reveals potential senotherapeutic targets in human and mouse cells |
| Description | Excessive accumulation of senescent cells contributes to a wide range of physiological declines and age-related pathologies. Senotherapy targeting senescent cell surface molecules represents an attractive approach to eliminate senescent cells with high specificity and accessibility. However, the comprehensive identification of surface proteins specific to cellular senescence is lacking. Here, we conducted quantitative mass spectrometry analysis on enriched cell surface proteins across eight experimental models of senescence induced by genotoxic, oxidative or proteasome stress in both fetal- and adult-derived murine and human cell types. Our analyses revealed pronounced changes in expression profiles of surfaceome during senescence, as well as universal alterations in functional features of cell mechanics and extracellular matrix remodeling. The cell type- and inducer-specific senescence signatures were also carried out by in-depth comparative analysis of surface proteomic profiling in cells undergoing distinct senescence conditions. Our investigation elucidated the heterogeneity of senescence, which is preferentially determined by respective cell type, from the perspective of the surfaceome. Moreover, we identified four cell surface proteins with extracellular epitopes, which were selectively expressed in senescent cells but not in proliferating cells, and elevated in various tissues of old mice and in the brain of an Alzheimer's disease mouse model. Our findings expand current understanding of senescence phenotypes that contribute to the intricate interactions between senescence cells and their extracellular milieu. The identified senescence surface biomarkers hold the potential to selectively capture and eliminate different senescent cell populations and subpopulations in the context of aging and age-related diseases in vivo. |
| HostingRepository | jPOST |
| AnnounceDate | 2025-01-26 |
| AnnouncementXML | Submission_2025-01-25_07:00:06.838.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Enzo Scifo |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | S-carboxamidomethyl-L-cysteine; N6-acetyl-L-lysine; alpha-amino acetylated residue; L-methionine sulfoxide |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-01-26 06:37:43 | ID requested | |
| ⏵ 1 | 2025-01-25 07:00:07 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: cellular senescence, aging, mass spectrometry, cell surface proteins, senotherapeutics |
Contact List
| Dr. Dan Ehninger | |
|---|---|
| lab head | |
| Enzo Scifo | |
| contact affiliation | DZNE |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002467/ |
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