PXD048920

PXD048920 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The proteomics effects of HSP60 chaperone deficiency in zebrafish larvae
Description	To understand the effects of Hsp60 deficiency in developing vertebrates, we generated CRISPR/Cas9-mediated hspd1 knockout zebrafish lines by targeting exon 2 to induce a frameshift mutation. We selected an allele with a 56 base pair deletion inducing a frameshift mutation leading to loss of protein functions. We examined the proteome changes in zebrafish larvae at 5 days post fertilization (DPF). Wildtype control and hspd1-/- larvae at 5dpf, were analyzed by TMT and nanoLC-MS/MS based proteomcis. For this purpose, we studied five pools from each genotype, and each pool consisted of five larvae.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:59:48.781.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD048920
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Johan Palmfeldt
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-26 05:20:14	ID requested
1	2024-09-27 03:18:37	announced
⏵ 2	2024-10-22 06:59:49	announced	2024-10-22: Updated project metadata.

Publication List

10.6019/PXD048920;
10.1016/j.molmet.2024.102009;
C, ö, mert C, Kj, æ, r-S, ø, rensen K, Hansen J, Carlsen J, Just J, Meaney BF, Ø, stergaard E, Luo Y, Oxvig C, Schmidt-Laursen L, Palmfeldt J, Fernandez-Guerra P, Bross P, HSP60 chaperone deficiency disrupts the mitochondrial matrix proteome and dysregulates cholesterol synthesis. Mol Metab, 88():102009(2024) [pubmed]

10.6019/PXD048920;

10.1016/j.molmet.2024.102009;

C, ö, mert C, Kj, æ, r-S, ø, rensen K, Hansen J, Carlsen J, Just J, Meaney BF, Ø, stergaard E, Luo Y, Oxvig C, Schmidt-Laursen L, Palmfeldt J, Fernandez-Guerra P, Bross P, HSP60 chaperone deficiency disrupts the mitochondrial matrix proteome and dysregulates cholesterol synthesis. Mol Metab, 88():102009(2024) [pubmed]

Keyword List

submitter keyword: larvae. LC-MS,HSP60, chaperone, zebrafish

submitter keyword: larvae. LC-MS,HSP60, chaperone, zebrafish

Contact List

Johan Palmfeldt
contact affiliation	Aarhus University, Denmark
contact email	johan.palmfeldt@clin.au.dk
lab head
Johan Palmfeldt
contact affiliation	Aarhus University Hospital
contact email	johan.palmfeldt@clin.au.dk
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD048920

PRIDE project URI

Repository Record List

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