PXD048919

PXD048919 is an original dataset announced via ProteomeXchange.

Title	Protein translation rate determines neocortical neuron fate
Description	The mammalian neocortex comprises an enormous diversity regarding cell types, morphology, and connectivity. In this work, we discover a post-transcriptional mechanism of gene expression regulation, protein translation, as a determinant of cortical neuron identity. We find specific upregulation of protein synthesis in the progenitors of later-born neurons and show that translation rates and concomitantly protein half-lives are inherent features of cortical neuron subtypes. In a small molecule screening, we identify Ire1a as a regulator of Satb2 expression and neuronal polarity. In the developing brain, Ire1a regulates global translation rates, coordinates ribosome traffic, and the expression of eIF4A1. Furthermore, we demonstrate that the Satb2 mRNA translation requires eIF4A1 helicase activity towards its 5’-untranslated region. Altogether, we show that cortical neuron diversity is generated by mechanisms operating beyond gene transcription, with Ire1a-safeguarded proteostasis serving as an essential regulator of brain development.
HostingRepository	PRIDE
AnnounceDate	2025-04-15
AnnouncementXML	Submission_2025-04-15_07:10:34.917.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Meierhofer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-26 03:32:58	ID requested
⏵ 1	2025-04-15 07:10:35	announced

10.1038/S41467-024-49198-W;

submitter keyword: neocortex, Satb2, Ire1alpha, brain development

Mateusz Ambrozkiewicz
contact affiliation	Group Leader Proteostasis Charité Universitätsmedizin Berlin, Germany
contact email	mateusz-cyryl.ambrozkiewicz@charite.de
lab head
David Meierhofer
contact affiliation	Mass Spectrometry Facility MPIMG
contact email	meierhof@molgen.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD048919

PRIDE project URI

• PRIDE
  1. PXD048919
     1. Label: PRIDE project
     2. Name: Protein translation rate determines neocortical neuron fate