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PXD048919

PXD048919 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein translation rate determines neocortical neuron fate
Description The mammalian neocortex comprises an enormous diversity regarding cell types, morphology, and connectivity. In this work, we discover a post-transcriptional mechanism of gene expression regulation, protein translation, as a determinant of cortical neuron identity. We find specific upregulation of protein synthesis in the progenitors of later-born neurons and show that translation rates and concomitantly protein half-lives are inherent features of cortical neuron subtypes. In a small molecule screening, we identify Ire1a as a regulator of Satb2 expression and neuronal polarity. In the developing brain, Ire1a regulates global translation rates, coordinates ribosome traffic, and the expression of eIF4A1. Furthermore, we demonstrate that the Satb2 mRNA translation requires eIF4A1 helicase activity towards its 5’-untranslated region. Altogether, we show that cortical neuron diversity is generated by mechanisms operating beyond gene transcription, with Ire1a-safeguarded proteostasis serving as an essential regulator of brain development.
HostingRepositoryPRIDE
AnnounceDate2025-04-15
AnnouncementXMLSubmission_2025-04-15_07:10:34.917.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDavid Meierhofer
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-26 03:32:58ID requested
12025-04-15 07:10:35announced
Publication List
10.1038/S41467-024-49198-W;
Keyword List
submitter keyword: neocortex, Satb2, Ire1alpha, brain development
Contact List
Mateusz Ambrozkiewicz
contact affiliationGroup Leader Proteostasis Charité Universitätsmedizin Berlin, Germany
contact emailmateusz-cyryl.ambrozkiewicz@charite.de
lab head
David Meierhofer
contact affiliationMass Spectrometry Facility MPIMG
contact emailmeierhof@molgen.mpg.de
dataset submitter
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Dataset FTP location
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