PXD048919 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protein translation rate determines neocortical neuron fate |
Description | The mammalian neocortex comprises an enormous diversity regarding cell types, morphology, and connectivity. In this work, we discover a post-transcriptional mechanism of gene expression regulation, protein translation, as a determinant of cortical neuron identity. We find specific upregulation of protein synthesis in the progenitors of later-born neurons and show that translation rates and concomitantly protein half-lives are inherent features of cortical neuron subtypes. In a small molecule screening, we identify Ire1a as a regulator of Satb2 expression and neuronal polarity. In the developing brain, Ire1a regulates global translation rates, coordinates ribosome traffic, and the expression of eIF4A1. Furthermore, we demonstrate that the Satb2 mRNA translation requires eIF4A1 helicase activity towards its 5’-untranslated region. Altogether, we show that cortical neuron diversity is generated by mechanisms operating beyond gene transcription, with Ire1a-safeguarded proteostasis serving as an essential regulator of brain development. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-15 |
AnnouncementXML | Submission_2025-04-15_07:10:34.917.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | David Meierhofer |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-26 03:32:58 | ID requested | |
⏵ 1 | 2025-04-15 07:10:35 | announced | |
Publication List
Keyword List
submitter keyword: neocortex, Satb2, Ire1alpha, brain development |
Contact List
Mateusz Ambrozkiewicz |
contact affiliation | Group Leader Proteostasis Charité Universitätsmedizin Berlin, Germany |
contact email | mateusz-cyryl.ambrozkiewicz@charite.de |
lab head | |
David Meierhofer |
contact affiliation | Mass Spectrometry Facility MPIMG |
contact email | meierhof@molgen.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048919
- Label: PRIDE project
- Name: Protein translation rate determines neocortical neuron fate