PXD048918

PXD048918 is an original dataset announced via ProteomeXchange.

Title	Proteomics effects of HSP60 deficiency in HEK cells, using an inducible transgenic HSP60 ATPase deficient mutant.
Description	To investigate the immediate effect of HSP60 dysfunction on mitochondrial functions, we induced the expression of a dominant-negative ATPase-deficient HSP60 mutant (HSP60-D423A) in HEK293 cells using tetracycline . Incorporation of HSP60-D423A subunits into HSP60 heptamer rings leads to dysfunction of the chaperonin complex. We titrated tetracycline levels to have a biologically relevant cellular model that can be monitored over time. Expression of the HSP60-D432A protein resulted in significantly reduced cell counts at 72 hours induction compared to uninduced and HSP60-WT co-expressing cells. To study the effects of onset of HSP60 deficiency, we monitored the proteomics changes using TMT based proteomics.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:09:57.898.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD048918
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Johan Palmfeldt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-26 03:22:57	ID requested
⏵ 1	2025-05-06 13:09:58	announced

10.1016/j.molmet.2024.102009;

C, ö, mert C, Kj, æ, r-S, ø, rensen K, Hansen J, Carlsen J, Just J, Meaney BF, Ø, stergaard E, Luo Y, Oxvig C, Schmidt-Laursen L, Palmfeldt J, Fernandez-Guerra P, Bross P, HSP60 chaperone deficiency disrupts the mitochondrial matrix proteome and dysregulates cholesterol synthesis. Mol Metab, 88():102009(2024) [pubmed]

10.6019/PXD048918;

submitter keyword: HSP60 deficiency, mitochondria, chaperone, differential protein expression

Johan Palmfeldt
contact affiliation	Aarhus University, Denmark
contact email	johan.palmfeldt@clin.au.dk
lab head
Johan Palmfeldt
contact affiliation	Aarhus University Hospital
contact email	johan.palmfeldt@clin.au.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD048918

PRIDE project URI

• PRIDE
  1. PXD048918
     1. Label: PRIDE project
     2. Name: Proteomics effects of HSP60 deficiency in HEK cells, using an inducible transgenic HSP60 ATPase deficient mutant.