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PXD048918

PXD048918 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics effects of HSP60 deficiency in HEK cells, using an inducible transgenic HSP60 ATPase deficient mutant.
DescriptionTo investigate the immediate effect of HSP60 dysfunction on mitochondrial functions, we induced the expression of a dominant-negative ATPase-deficient HSP60 mutant (HSP60-D423A) in HEK293 cells using tetracycline . Incorporation of HSP60-D423A subunits into HSP60 heptamer rings leads to dysfunction of the chaperonin complex. We titrated tetracycline levels to have a biologically relevant cellular model that can be monitored over time. Expression of the HSP60-D432A protein resulted in significantly reduced cell counts at 72 hours induction compared to uninduced and HSP60-WT co-expressing cells. To study the effects of onset of HSP60 deficiency, we monitored the proteomics changes using TMT based proteomics.
HostingRepositoryPRIDE
AnnounceDate2025-05-06
AnnouncementXMLSubmission_2025-05-06_13:09:57.898.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD048918
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJohan Palmfeldt
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-26 03:22:57ID requested
12025-05-06 13:09:58announced
Publication List
10.1016/j.molmet.2024.102009;
C, ö, mert C, Kj, æ, r-S, ø, rensen K, Hansen J, Carlsen J, Just J, Meaney BF, Ø, stergaard E, Luo Y, Oxvig C, Schmidt-Laursen L, Palmfeldt J, Fernandez-Guerra P, Bross P, HSP60 chaperone deficiency disrupts the mitochondrial matrix proteome and dysregulates cholesterol synthesis. Mol Metab, 88():102009(2024) [pubmed]
10.6019/PXD048918;
Keyword List
submitter keyword: HSP60 deficiency, mitochondria, chaperone, differential protein expression
Contact List
Johan Palmfeldt
contact affiliationAarhus University, Denmark
contact emailjohan.palmfeldt@clin.au.dk
lab head
Johan Palmfeldt
contact affiliationAarhus University Hospital
contact emailjohan.palmfeldt@clin.au.dk
dataset submitter
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Dataset FTP location
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