PXD048918 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics effects of HSP60 deficiency in HEK cells, using an inducible transgenic HSP60 ATPase deficient mutant. |
Description | To investigate the immediate effect of HSP60 dysfunction on mitochondrial functions, we induced the expression of a dominant-negative ATPase-deficient HSP60 mutant (HSP60-D423A) in HEK293 cells using tetracycline . Incorporation of HSP60-D423A subunits into HSP60 heptamer rings leads to dysfunction of the chaperonin complex. We titrated tetracycline levels to have a biologically relevant cellular model that can be monitored over time. Expression of the HSP60-D432A protein resulted in significantly reduced cell counts at 72 hours induction compared to uninduced and HSP60-WT co-expressing cells. To study the effects of onset of HSP60 deficiency, we monitored the proteomics changes using TMT based proteomics. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-06 |
AnnouncementXML | Submission_2025-05-06_13:09:57.898.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD048918 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Johan Palmfeldt |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-26 03:22:57 | ID requested | |
⏵ 1 | 2025-05-06 13:09:58 | announced | |
Publication List
10.1016/j.molmet.2024.102009; |
C, ö, mert C, Kj, æ, r-S, ø, rensen K, Hansen J, Carlsen J, Just J, Meaney BF, Ø, stergaard E, Luo Y, Oxvig C, Schmidt-Laursen L, Palmfeldt J, Fernandez-Guerra P, Bross P, HSP60 chaperone deficiency disrupts the mitochondrial matrix proteome and dysregulates cholesterol synthesis. Mol Metab, 88():102009(2024) [pubmed] |
10.6019/PXD048918; |
Keyword List
submitter keyword: HSP60 deficiency, mitochondria, chaperone, differential protein expression |
Contact List
Johan Palmfeldt |
contact affiliation | Aarhus University, Denmark |
contact email | johan.palmfeldt@clin.au.dk |
lab head | |
Johan Palmfeldt |
contact affiliation | Aarhus University Hospital |
contact email | johan.palmfeldt@clin.au.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048918
- Label: PRIDE project
- Name: Proteomics effects of HSP60 deficiency in HEK cells, using an inducible transgenic HSP60 ATPase deficient mutant.