PXD048915
PXD048915 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mechanism of Jianxin granules in the Treatment of Heart Failure Based on Proteomics and Metabolomics |
Description | The study aimed to investigate the mechanisms of Jianxin (JX) granules, a Traditional Chinese Medicine formulation, in the treatment of heart failure (HF) using metabolomic profiling. The HF model was established by ligation of left coronary artery. The successfully modeled rats were randomly divided into three groups: the model group, the JX granules group, and Sacubitril/Valsartan (S/V) group. Four weeks after treatment, LV tissue samples were collected. Proteomics was used to identify the differentially expressed proteins and potential pathways. The differential expressed proteins (DEPs) were obtained with Tandem Mass Tags approach. As compared to the control group, 150 DEPs were identified in model rats, comprising 112 up-regulated and 38 down-regulated proteins. Notably, in the JX granules group, 25 DEPs were identified compared to the model rats, encompassing 18 up-regulated and 7 down-regulated proteins. In comparison with the control group, 228 DEPs in the JX granules group were obtained, which comprised of 128 up-regulated and 100 down-regulated proteins. As compared with the control group, BP in the model group was mainly enriched in humoral immune response, positive regulation of apoptotic cell clearance, and the response to metal ions; Meanwhile, CC was mainly related to contractile and stress fibers, actin filaments, and extracellular space; MF was mainly enriched in the serine-type peptidase activity, actin binding, and peptidase activity. Furthermore, DEPs indicated by KEGG analysis were primarily linked to pathways such as the complement and coagulation cascades, regulation of the actin cytoskeleton, and adrenergic signaling. In contrast to the model group, BP in JX granules group was primarily associated with processes involving nucleosome organization, chromatin assembly or disassembly, DNA conformation change and small molecule catabolic processes; CC was primarily related to the protein-DNA complex, mitochondrion, intracellular, nucleosome, cytoplasm; MF displayed significant enrichment in protein dimerization and homodimerization activity, catalase activity, chromatin and nucleosomal DNA binding. Considering KEGG enrichment information, DEPs in the JX granules group were mainly enriched in metabolic pathways and ribosome pathway. The key differential genes, such as triosephosphate isomerase 1 (TPI1), lactate dehydrogenase B (LDHB), pyruvate kinase M (PKM), protein kinase B (Akt), and lactate dehydrogenase A (LDHA) were identified. The vital pathways including carbon metabolism, the PI3K-Akt signaling pathway, pyruvate metabolism, and HIF-1α signaling pathway were obtained. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-07 |
AnnouncementXML | Submission_2024-02-07_03:02:01.945.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | pan yichun |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | S-carboxamidoethyl-L-cysteine |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-01-26 02:16:28 | ID requested | |
1 | 2024-01-26 14:05:59 | announced | |
⏵ 2 | 2024-02-07 03:02:02 | announced | 2024-02-07: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Traditional Chinese Medicine, myocardium,Heart failure |
JX granules |
Proteomics |
rats |
Contact List
Ouyang Qiufang | |
---|---|
contact affiliation | Ultrasound Department, The Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine |
contact email | torrent_100@163.com |
lab head | |
pan yichun | |
contact affiliation | company |
contact email | 1986348844@qq.com |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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