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PXD048903

PXD048903 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTargeting PILRα, an immune checkpoint mediating T cell suppression, for cancer immunotherapy
DescriptionOne of the most successful therapies in cancer treatment involves immune checkpoint blockade using the anti-programmed death-1 (anti-PD-1)/programmed death-ligand 1 (PD-L1) antibody, which targets the interaction between tumors and T cells. However, the overall response rate is < 30% in patients with cancer. Therefore, there is an urgent need to identify additional immune checkpoints that can modulate T-cell function. In this study, we identified the tumor cell-expressed paired Ig-like type 2 receptor α (PILRα) as an immune suppressor that targets T cells, using a high-throughput screening approach. PILRα suppresses T-cell activation, proliferation, and effector function in both cultured cells and mouse xenograft and allograft models. It specifically targets CD99, a T cell surface antigen, to suppress the ZAP70/NFAT/IL2/JAK/STAT signaling in T cells. The cluster of O-glycosylated serine (Ser)/threonine (Thr) residues within the stalk region plays a critical role in PILRα–CD99 interactions, thereby influencing the immunosuppressive function of PILRα. Blocking interactions between PILRα and CD99 using an anti-PILRα antibody that targets the stalk region potently enhances T cell antitumor immunity and suppresses tumor growth. Additionally, when combined anti-PILRα with anti-PD-1 antibodies, synergistic effects on tumor suppression were observed. Notably, PILRα is highly expressed in various human cancers, and its expression levels serve as a predictive marker for poor prognosis in patients with cancer. Taken together, our results unveil PILRα as an immune checkpoint and highlight the promising therapeutic potential of targeting PILRα in clinical cancer immunotherapy.
HostingRepositoryiProX
AnnounceDate2024-01-25
AnnouncementXMLSubmission_2025-03-13_18:36:39.737.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYaohui He
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-25 18:15:55ID requested
12025-03-13 18:36:40announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: PILRα, immune checkpoint, T-cell, CD99, cancer immunotherapy
Contact List
Wen Liu
contact affiliationXiamen University
contact emailw2liu@xmu.edu.cn
lab head
Yaohui He
contact affiliationXiamen University
contact emailyhhe@xmu.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI