PXD048869

PXD048869 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation of the compartmentalized PKA substrate TAF15 regulates RNA-Protein interactions
Description	Spatiotemporal-controlled second messengers alter molecular interactions of central signaling nodes for ensuring physiological signal transmission. One prototypical second messenger molecule which modulates kinase signal transmission is the cyclic-adenosine monophosphate (cAMP). The main proteinogenic cellular effectors of cAMP are compartmentalized protein kinase A (PKA) complexes. Their cell-type specific compositions precisely coordinate substrate phosphorylation and proper signal propagation which is indispensable for numerous cell-type specific functions. Here we present evidence that TAF15, which is implicated in the etiology of amyotrophic lateral sclerosis, represents a novel nuclear PKA substrate. In cross-linking and immunoprecipitation experiments (iCLIP) we showed that TAF15 phosphorylation alters the binding to target transcripts related to mRNA maturation, splicing and protein-binding related functions. TAF15 appears to be one of multiple PKA substrates that undergo RNA-binding dynamics upon phosphorylation. We observed that the activation of the cAMP-PKA signaling axis caused a change in the composition of a collection of RNA species that interact with TAF15. This observation appears to be a broader principle in the regulation of molecular interactions, as we identified a significant enrichment of RNA-binding proteins within endogenous PKA complexes. We assume that phosphorylation of RNA-binding domains adds another layer of regulation to binary protein-RNAs interactions with consequences to RNA features including binding specificities, localization, abundance and composition.
HostingRepository	PRIDE
AnnounceDate	2024-04-04
AnnouncementXML	Submission_2024-04-04_02:22:40.284.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Omar Torres
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-25 02:21:30	ID requested
⏵ 1	2024-04-04 02:22:40	announced

10.1007/S00018-024-05204-4;

submitter keyword: cAMP, TAF15,PKA, phosphorylation, RNA

Eduard Stefan
contact affiliation	1-Tyrolean Cancer Research Institute (TKFI), Innrain 66, A-6020 Innsbruck, Austria 2-Institute of Molecular Biology and Center for Molecular Biosciences, University of Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, Austria.
contact email	eduard.stefan@uibk.ac.at
lab head
Omar Torres
contact affiliation	Institute Medical Biochemistry, Medical University Innsbruck, Innrain 80-82, A-6020, Innsbruck, Austria
contact email	omar.torres-quesada@i-med.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD048869

PRIDE project URI

• PRIDE
  1. PXD048869
     1. Label: PRIDE project
     2. Name: Phosphorylation of the compartmentalized PKA substrate TAF15 regulates RNA-Protein interactions