PXD048857

PXD048857 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-based LC-MS/MS analysis of benign prostatic hyperplasia prostate tissues with different stroma/epithelium ratio
Description	To investigate proteins potentially involved in the survival of prostate epithelial cells, we initially collected prostate tissues from 32 BPH patients. Subsequently, we conducted immunohistochemistry (IHC) staining using an anti-alpha-smooth muscle actin (α-SMA) antibody, recognized as a marker for smooth muscle cells and myofibroblasts. We delineated the areas of the stroma, glandular epithelium, and glandular lumen based on this staining. We digitized and conducted subsequent analysis of the relative percentage of different tissue components, including the stroma/epithelium ratio (S/E). Specimens were ranked based on the S/E ratio, leading to the categorization of the 9 specimens with the lowest S/E into group 1 (Epithelium-hi), where the epithelial components may have more significant roles in BPH development. Conversely, the 9 specimens with the highest S/E were placed in group 2 (Epithelium-lo). Within each group, we further subdivided them into 3 subgroups labeled as A through E. Subsequently, we employed isobaric tags for relative and absolute quantitation (iTRAQ)-based liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify the differentially expressed proteins (DEPs) between the two main groups.
HostingRepository	iProX
AnnounceDate	2024-01-24
AnnouncementXML	Submission_2024-03-06_00:04:09.086.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhifu Liu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-24 18:42:09	ID requested
1	2024-01-24 18:42:28	announced
⏵ 2	2024-03-06 00:04:09	announced	2024-03-06: Update information.

Liu Z, Li S, Chen S, Sheng J, Li Z, Lv T, Yu W, Fan Y, Wang J, Liu W, Hu S, Jin J, YAP-mediated GPER signaling impedes proliferation and survival of prostate epithelium in benign prostatic hyperplasia. iScience, 27(3):109125(2024) [pubmed]

submitter keyword: Benign prostatic hyperplasia (BPH), Prostate epithelial cell, Prostate stromal cell, Proliferation-apoptosis balance.

Jie Jin
contact affiliation	Department of Urology, Peking University First Hospital
contact email	jinjie@vip.163.com
lab head
Zhifu Liu
contact affiliation	Department of Urology, Peking University First Hospital
contact email	zfliupku@126.com
dataset submitter

iProX dataset URI