PXD048847

PXD048847 is an original dataset announced via ProteomeXchange.

Title	Interacting proteins on lncRNA SMANTIS in THP-1 cells
Description	The lncRNA SMANTIS (formerly known as MANTIS, AK125871, n342419) functions as a regulator of cell function in endothelial cells and for adhesion to monocytes. Here, we investigated the function of SMANTIS in THP-1 monocytes. By using an antisense oligonucleotide pulldown approach followed by LC/MS, the protein interaction partners of SMANTIS in THP-1 cells were identified. This approach was performed in NTC (non-targeting control) THP-1, where SMANTIS is present, and in SMANTIS-LentiCRISPR KO THP-1, where SMANTIS was removed. The data revealed that a lot of RNA-binding proteins interact with SMANTIS.
HostingRepository	PRIDE
AnnounceDate	2025-01-07
AnnouncementXML	Submission_2025-01-07_04:11:30.688.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilka Wittig
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-24 10:41:38	ID requested
⏵ 1	2025-01-07 04:11:31	announced

Weiss LM, Warwick T, Zehr S, G, ü, nther S, Wolf S, Schmachtel T, Izquierdo Ponce J, P, á, lfi K, Teichmann T, Schneider A, St, ö, tzel J, Knapp S, Weigert A, Savai R, Rieger MA, Oellerich T, Wittig I, Oo JA, Brandes RP, Leisegang MS, RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions. Commun Biol, 7(1):1131(2024) [pubmed]

10.1038/s42003-024-06794-2;

submitter keyword: Monocytes, complex remodeling, lncRNA, protein-RNA complexes

Matthias S. Leisegang
contact affiliation	Institute for Cardiovascular Physiology, Faculty of Medicine, Goethe-University; Frankfurt am Main, Germany
contact email	Leisegang@vrc.uni-frankfurt.de
lab head
Ilka Wittig
contact affiliation	Functional Proteomics, Goethe University, Frankfurt am Main , Germany
contact email	wittig@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD048847

PRIDE project URI

• PRIDE
  1. PXD048847
     1. Label: PRIDE project
     2. Name: Interacting proteins on lncRNA SMANTIS in THP-1 cells