PXD048815

PXD048815 is an original dataset announced via ProteomeXchange.

Title	Proteomics analyses of small extracellular vesicles of aqueous humor: identification and validation of GAS6 and SPP1 as glaucoma markers during cataracts surgery
Description	A high percentage of vision loss and blindness worldwide are due to glaucoma and cataracts ocular diseases. Small extracellular vesicles (sEVs) are small lipidic vesicles involved in transport and cell-to-cell communication, released into different body fluids including eye’s aqueous humor. The identification and characterization of the protein content of small extracellular vesicles in ocular pathologies has not been well elucidated yet. To that end, a by quantitative proteomics approach using Tandem Mass Tag (TMT) 10-Plex was performed to analyze the protein content of extracellular vesicles from patients´ aqueous humors with cataracts and glaucoma compared to healthy individuals (ICL). Labeled peptides were analyzed on an Orbitrap Exploris 480 equipped with FAIMS Pro Duo interface. Then, western blot (WB) and ELISA analyses using aqueous humor samples from ICL, cataracts, and glaucoma patients were performed for validation. From the TMT 10-plex analysis of sEVs, a total of 828 peptides and 192 proteins were identified and quantified. After data analysis with R program, 14 significantly dysregulated proteins from sEVs of aqueous humor in cataracts and 11 in glaucoma showed a fold change ≥1.5. Then, the dysregulation of 10 candidate dysregulated proteins was confirmed by WB and ELISA using directly aqueous humor samples. In addition, GAS6 and SPP1 were found to possess a high diagnostic ability of glaucoma patients. In conclusion, the proteomics analysis of sEVs from aqueous humor samples allowed the identification of GAS6 and SPP1 as glaucoma biomarkers, which in combination allowed for discriminating glaucoma patients from control individuals with an area under the curve (AUC) of 76.1%, and a sensitivity of 65.6%, and a specificity of 87.7%.
HostingRepository	PRIDE
AnnounceDate	2024-11-04
AnnouncementXML	Submission_2024-11-04_06:44:23.998.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana Montero Calle
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-23 16:26:51	ID requested
⏵ 1	2024-11-04 06:44:24	announced

Dataset with its publication pending

submitter keyword: glaucoma diagnosis, proteomics, aqueous humor,glaucoma, small extracellular vesicles, ELISA, cataracts

Rodrigo Barderas
contact affiliation	Chronic Disease Programme, UFIEC, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain
contact email	r.barderasm@isciii.es
lab head
Ana Montero Calle
contact affiliation	Instituto de Salud Carlos III
contact email	ana.monteroc@isciii.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048815
     1. Label: PRIDE project
     2. Name: Proteomics analyses of small extracellular vesicles of aqueous humor: identification and validation of GAS6 and SPP1 as glaucoma markers during cataracts surgery