PXD048811

PXD048811 is an original dataset announced via ProteomeXchange.

Title	Electron Capture vs. Transfer Dissociation for Site Determination of Tryptic Peptide Tyrosine Sulfation: Direct Detection of Fibrinogen Sulfation Sites and Identification of Novel Isobaric Interferences
Description	Tyrosine sulfation is an understudied posttranslational modification (PTM) of crucial biological significance. Conventional liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods are unable to directly identify sulfation sites due to the high lability of the sulfate PTM. In this study, we were able to directly detect and localize tyrosine sulfation sites in a proteomics workflow. To meet that objective, we combined bottom-up nanoflow LC-beam-type CID (nanoLC-HCD) with conventional “MSFragger-Labile” analysis, nanoLC-electron transfer with supplemental HCD (EThcD) MS/MS, and LC-electron capture dissociation (ECD) MS/MS for the characterization of bovine fibrinogen sulfopeptide candidates from both purified protein and from plasma.
HostingRepository	PRIDE
AnnounceDate	2024-06-21
AnnouncementXML	Submission_2024-06-20_19:19:09.559.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD048811
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Menatallah Youssef
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	O4'-sulfo-L-tyrosine
Instrument	Orbitrap Fusion Lumos; Bruker Daltonics solarix series

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-23 11:03:00	ID requested
⏵ 1	2024-06-20 19:19:09	announced

10.1021/ACS.JPROTEOME.4C00072;

10.6019/PXD048811;

submitter keyword: post-translational modifications, mass spectrometry, direct determination,Tyrosine sulfation, proteomics.

Kristina Hakansson
contact affiliation	Department of Chemistry, University of Michigan, Ann Arbor, MI, United States
contact email	kicki@umich.edu
lab head
Menatallah Youssef
contact affiliation	Department of Chemistry, University of Michigan
contact email	menaty@umich.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048811
     1. Label: PRIDE project
     2. Name: Electron Capture vs. Transfer Dissociation for Site Determination of Tryptic Peptide Tyrosine Sulfation: Direct Detection of Fibrinogen Sulfation Sites and Identification of Novel Isobaric Interferences