PXD048810
PXD048810 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | FTIR, XPol, and LC-MS confirm endogenous collagen in Edmontosaurus fossil bone C4PR_LIV |
| Description | Reports of proteins in fossilized bones have ignited an on-going debate because previously it was generally assumed that fossilization results in destruction of all organic components. We combine Attenuated Total Reflection (ATR) Fourier Transform InfraRed (FTIR) spectroscopy, cross-polarized light microscopy (XPol), and two variations of Liquid Chromatography Mass Spectrometry (LC-MS), to detect and verify endogenous collagen protein in fossilized bone. Data from an Edmontosaurus dinosaur excavated from Upper Cretaceous strata of the South Dakota Hell Creek Formation were compared with that from thermally decayed modern turkey (Meleagris) bone. The four methods show different evidence for collagen presence in the fossilized bone. ATR-FTIR spectra show a signal for carbonyl (C=O) groups, a repeat moiety in protein amino acid sequences. XPol shows the birefringence of collagen within Meleagris, as well as Edmontosaurus. LC-MS bottom-up proteomics showed m/z agreement between multiple ions from trypsin-digested samples and identified peptide sequences thus far only identified in a hadrosaur. Tandem LC-MS unambiguously identified hydroxyproline, a unique collagen-indicator amino acid, in acid-digested samples from the same. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-03-24 |
| AnnouncementXML | Submission_2025-03-24_04:20:01.263.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD048810 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Philip Brownridge |
| SpeciesList | scientific name: Dinosauria; NCBI TaxID: 436486; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Meleagris gallopavo (Common turkey); NCBI TaxID: 9103; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-01-23 09:44:48 | ID requested | |
| ⏵ 1 | 2025-03-24 04:20:02 | announced |
Publication List
| 10.6019/PXD048810; |
| Tuinstra L, Thomas B, Robinson S, Pawlak K, Elezi G, Faull KF, Taylor S, Fossil Bone. Anal Chem, 97(5):2618-2628(2025) [pubmed] |
| 10.1021/acs.analchem.4c03115; |
Keyword List
| submitter keyword: dinosaur, collagen, fossil |
Contact List
| Stephen Taylor | |
|---|---|
| contact affiliation | Department of Electrical Engineering and Electronics University of Liverpool Liverpool |
| contact email | ee63@liverpool.ac.uk |
| lab head | |
| Philip Brownridge | |
| contact affiliation | University of Liverpool |
| contact email | philipjb@liv.ac.uk |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD048810 |
| PRIDE project URI |
Repository Record List
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