PXD048801

PXD048801 is an original dataset announced via ProteomeXchange.

Title	Poly (ADP-ribose) polymerase in yeasts: characterization and involvement in telomere maintenance
Description	Poly (ADP-ribose) polymerases (PARPs) are specific enzymes catalyzing the post-translational addition of ADP-ribose moieties to proteins. In most eukaryotic cells, their primary protein targets are involved in DNA recombination, repair, and chromosome maintenance. Even though this group of enzymes is quite common in both eukaryotes and prokaryotes, no PARP homologs have been described so far in ascomycetous yeasts, leaving their potential roles in this group of organisms unexplored. Analysis of transcriptome of telomerase-deficient cells of the yeast Yarrowia lipolytica resulted in a serendipitous identification of the gene PYL1 encoding the first candidate of PARP homolog in yeasts. Here, we show that Pyl1 protein is a functional PARP that undergoes auto-PARylation and we identified several of its protein targets in vivo. The fact that the major targets of PARylation are both subunits of the Ku70/Ku80 complex and that overexpression of PYL1 leads to elongation of telomeres support the role of Pyl1 in the adaptation of Y. lipolytica cells to the genomic stress in general and loss of telomerase in particular. Interestingly, Δpyl1 mutants did not exhibit increased sensitivity to DNA-damaging agents known to activate PARylation in mammalian cells, indicating that compared with mammalian PARPs Pyl1p plays a different role in the maintenance of genome stability. Based on our observations, we propose Pyl1 and its homologs we identified in other yeast species represent a distinct class of PARPs thus substantiating a more detailed investigation of their roles in these organisms.
HostingRepository	PRIDE
AnnounceDate	2025-09-15
AnnouncementXML	Submission_2025-09-14_16:16:47.123.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katerina Hanakova
SpeciesList	scientific name: Yarrowia lipolytica (Candida lipolytica); NCBI TaxID: 4952;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-23 05:39:16	ID requested
⏵ 1	2025-09-14 16:16:48	announced

Sep, š, iov, á R, Proch, á, zkov, á K, Č, erven, á, k F, Majer, č, í, k D, Han, á, kov, á K, Lattov, á E, Hajikazemi M, Zdr, á, hal Z, Vir, á, gov, á S, Brz, á, č, ov, á Z, Paeschke K, Nosek J, Tom, á, š, ka Ľ, Poly (ADP-ribose) polymerase in yeasts: characterization and involvement in telomere maintenance. Nucleic Acids Res, 53(17):(2025) [pubmed]

10.1093/nar/gkaf837;

submitter keyword: PARP,Yarrowia lipolytica, LC-MS/MS

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
Katerina Hanakova
contact affiliation	CEITEC MU
contact email	katerina.hanak@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD048801

PRIDE project URI

• PRIDE
  1. PXD048801
     1. Label: PRIDE project
     2. Name: Poly (ADP-ribose) polymerase in yeasts: characterization and involvement in telomere maintenance