PXD048798 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | FP-biotin enrichment of serine hydrolases in Enterococcus faecium |
Description | In our study, we employed activity-based protein profiling (ABPP), a technique that uses specialized inhibitors to identify active serine hydrolases in different strains of E. faecium (clade A1 and A2) and E. lactis under various growth conditions. Serine hydrolases, a large and diverse family of enzymes that include established drug targets like penicillin-binding proteins, have other less-studied subfamilies. In addition to fluorescent, gel-based profiling, we used a biotin-tagged fluorophosphonate probe for the enrichment and identification of serine hydrolase enzymes via streptavidin enrichment and liquid chromatography/mass spectrometry analysis. This led to the discovery of 11 largely unexplored potential targets (including α,β-hydrolases, SGNH-hydrolases, phospholipases, amidases, and peptidases) that could be exploited for drug developmentagainst the vancomycin-resistant E. faecium strain E745. |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-22 |
AnnouncementXML | Submission_2024-06-22_08:33:19.562.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jeanette, Slettnes Grunnvåg |
SpeciesList | scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Enterococcus faecium; NCBI TaxID: 1352; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-23 03:06:24 | ID requested | |
⏵ 1 | 2024-06-22 08:33:20 | announced | |
Publication List
Keyword List
submitter keyword: LC-MSMS,Enterococcus faecium, Activity-based protein profiling, FP-biotin, serine hydrolases |
Contact List
Christian Lentz |
contact affiliation | Host-Microbe interactions group, the department of medical biology, UiT - The Arctic University of Norway, Tromsø, Norway. |
contact email | christian.s.lentz@uit.no |
lab head | |
Jeanette, Slettnes Grunnvåg |
contact affiliation | UiT - The Arctic University of Norway |
contact email | jeanette.grunnvag@uit.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048798
- Label: PRIDE project
- Name: FP-biotin enrichment of serine hydrolases in Enterococcus faecium