PXD048796

PXD048796 is an original dataset announced via ProteomeXchange.

Title	Molecular and cellular adaptations of the mammary gland regulating milk constitutes and immunity of heat stressed dairy cows
Description	The molecular and cellular mechanisms underlying the adaption of the mammary gland (MG) to increased ambient heat, and which account for changes in milk composition and immunity are not known yet. This study aimed to elucidate the expression of MG proteins and differences in milk composition and ruminal short-chain fatty acid concentrations of dairy cows experiencing 7 days of heat stress (28°C, THI = 76), pair-feeding (PF) or ad libitum feeding (CON) at thermoneutrality (16°C, THI = 60; each n=10). The feed intake of PF cows were matched to the intake of HS cows. The portion of ruminal acetate, the acetate:propionate ratio, and milk urea concentration were greater whereas milk protein yield and lactose yield were lower in HS than CON cows on day 3 and 6 of the challenge, whilst energy and fat corrected milk were lower in HS than CON and PF cows. The proteome analysis revealed induced bacterial invasion of epithelial cells, leukocyte transendothelial migration, reduction of the pyruvate and carbon metabolism, and platelet activation in the MG of HS compared to CON and/or PF cows. These results highlight adaptive metabolic and immune responses to mitigate the negative effects of ambient heat in the mammary gland.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_12:56:32.428.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dirk Albrecht
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-23 02:22:50	ID requested
⏵ 1	2025-05-06 12:56:33	announced

10.1021/acs.jafc.4c03879;

Koch F, Albrecht D, Albrecht E, Hansen C, Kuhla B, Novel Perspective on Molecular and Cellular Adaptations of the Mammary Gland-Regulating Milk Constituents and Immunity of Heat-Stressed Dairy Cows. J Agric Food Chem, 72(37):20286-20298(2024) [pubmed]

submitter keyword: hyperthermia, adaptation, inflammation, dairy cows, udder

Björn Kuhla
contact affiliation	Institute for Farm Animal Biology (FBN), Dummerstorf, Germany
contact email	b.kuhla@fbn-dummerstorf.de
lab head
Dirk Albrecht
contact affiliation	EMAU
contact email	dirkalbr@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD048796

PRIDE project URI

• PRIDE
  1. PXD048796
     1. Label: PRIDE project
     2. Name: Molecular and cellular adaptations of the mammary gland regulating milk constitutes and immunity of heat stressed dairy cows