PXD048789

PXD048789 is an original dataset announced via ProteomeXchange.

Title	Effect of 2D and 3D cell culture system on trophoblast extracellular vesicles physio-chemical characteristics and potency
Description	In this project, trophoblast cell analogue human chorionic carcinoma cell line was cultivated in both conventional monolayer culture (2D) and as spheroids in suspension cultures (3D) and how the cell growth environment affects the physical and biochemical properties of EVs produced by them was studied. Importantly, proteomic cargo profile of EVs derived from 2D and 3D cultures was compared. There was substantial differences in the proteomic cargo profile and potency of EVs derived from two culture systems. Findings of this study will help in selecting an EV production platform, especially for assessing the functional and therapeutic potential of EVs.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:38:33.242.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SUBHASHINI MUHANDIRAM MUHANDIRAM RALALAGE
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-22 23:12:03	ID requested
1	2024-05-08 01:56:00	announced
⏵ 2	2024-10-22 06:38:33	announced	2024-10-22: Updated project metadata.

10.3389/FCELL.2024.1382552;

submitter keyword: trophoblast cells,Extracellular vesicles, embryo implantation, 2D culture, 3D culture

Alireza Fazeli
contact affiliation	Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences
contact email	alireza.fazeli@emu.ee
lab head
SUBHASHINI MUHANDIRAM MUHANDIRAM RALALAGE
contact affiliation	Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences
contact email	soomuhandiram2@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD048789

PRIDE project URI

• PRIDE
  1. PXD048789
     1. Label: PRIDE project
     2. Name: Effect of 2D and 3D cell culture system on trophoblast extracellular vesicles physio-chemical characteristics and potency