PXD048774

PXD048774 is an original dataset announced via ProteomeXchange.

Title	Comparative analysis of retroviral Gag-host cell interactions in the nucleus
Description	Retroviruses exploit a variety of host proteins to assemble and release virions from infected cells. To date, most studies that examined possible interacting partners of retroviral Gag proteins focused on host proteins that localize primarily to the cytoplasm or plasma membrane. Given the recent findings that several full-length Gag proteins localize to the nucleus, identifying the Gag-nuclear interactome has high potential for novel findings that reveal previously unknown host processes. This dataset contains results from a mass spectrometry approach using affinity-tagged (His6) HIV-1 and RSV Gag proteins mixed with nuclear extracts, and identifies potential binding partners of HIV-1 and RSV Gag involved in several nuclear processes, including transcription, splicing, RNA modification, and chromatin structure. Although a subset of host proteins interacted with both Gag proteins, there were also unique host proteins belonging to each interactome dataset. These results provide a strong premise for future functional studies to investigate roles for these nuclear host factors that may have shared functions in the biology of both retroviruses, as well as functions specific to RSV and HIV-1, given their distinctive hosts and molecular pathology.
HostingRepository	PRIDE
AnnounceDate	2024-07-03
AnnouncementXML	Submission_2024-07-03_02:00:54.428.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leslie Parent
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-22 13:11:21	ID requested
⏵ 1	2024-07-03 02:00:55	announced

10.1186/s12977-024-00645-y;

Lambert GS, Rice BL, Maldonado RJK, Chang J, Parent LJ, Comparative analysis of retroviral Gag-host cell interactions: focus on the nuclear interactome. Retrovirology, 21(1):13(2024) [pubmed]

submitter keyword: proteomics, mass spectrometry, Rous sarcoma virus, HIV-1,retroviruses

Leslie Joan Parent
contact affiliation	Departments of Medicine, Microbiology and Immunology Penn State College of Medicine Hershey, PA
contact email	ljp5@psu.edu
lab head
Leslie Parent
contact affiliation	Penn State College of Medicine
contact email	ljp5@psu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD048774

PRIDE project URI

• PRIDE
  1. PXD048774
     1. Label: PRIDE project
     2. Name: Comparative analysis of retroviral Gag-host cell interactions in the nucleus