PXD048720

PXD048720 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of a rat streptozotocin model shows dysregulated biological pathways implicated in Alzheimer’s Disease
Description	Alzheimer’s Disease (AD) is an age-related neurodegenerative disorder characterized by progres-sive memory loss and cognitive impairment, affecting 35 million individuals worldwide. Intracer-ebroventricular (ICV) injection of low to moderate doses of STZ in adult male Wistar rats can re-produce classical physiopathological hallmarks of AD. This biological model is known as ICV-STZ. Most studies are focused on the description of behavioral and morphological aspects of the ICV-STZ model. However, the knowledge regarding the molecular aspects of the ICV-STZ model is still in-cipient. Therefore, this work is a first attempt to provide a wide proteome description of the ICV-STZ model based on Mass spectrometry (MS). To achieve that, samples from pre-frontal cortex (PFC) and hippocampus (HPC) of the ICV-STZ model and control (wild-type) were used. Differ-ential protein abundance, pathway, and network analysis was performed based on the protein identification and quantification of the samples. Our analysis revealed dysregulated biological pathways implicated in early stages of Late-Onset Alzheimer’s Disease (LOAD) based on differen-tially abundant proteins (DAPs). Some of these DAPs had their mRNA expression further inves-tigated through qRT-PCR. Our results shed light on the AD onset and demonstrate the ICV-STZ as a valid model for LOAD proteome description.
HostingRepository	PRIDE
AnnounceDate	2024-06-22
AnnouncementXML	Submission_2024-06-22_08:43:05.939.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Esdras Matheus Gomes da Silva
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-19 12:48:48	ID requested
⏵ 1	2024-06-22 08:43:06	announced

da Silva EMG, Fischer JSG, Souza ILS, Andrade ACC, Souza LCE, Andrade MK, Carvalho PC, Souza RLR, Vital MABF, Passetti F, Proteomic Analysis of a Rat Streptozotocin Model Shows Dysregulated Biological Pathways Implicated in Alzheimer's Disease. Int J Mol Sci, 25(5):(2024) [pubmed]

10.3390/ijms25052772;

submitter keyword: Pre-frontal cortex (PFC)

Hippocampus (HPC)

Streptozotocin

Fabio Passetti
contact affiliation	Instituto Carlos Chagas, FIOCRUZ, Curitiba, PR, Brazil
contact email	fabio.passetti@fiocruz.br
lab head
Esdras Matheus Gomes da Silva
contact affiliation	Fiocruz
contact email	matheusdras.silva@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048720
     1. Label: PRIDE project
     2. Name: Proteomic analysis of a rat streptozotocin model shows dysregulated biological pathways implicated in Alzheimer’s Disease