PXD048716

PXD048716 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Nontargeted plasma proteomics analysis uncovers candidate biomarkers for renal disease and pulmonary hypertension in patients with sickle cell disease
Description	Fifteen microliters of plasma from sickle cell disease patients, and pooled samples, were diluted with 5% deoxycholate and 10 mM DTT, followed by heating at 80 degC for 30 min, alkylation with 25 mM iodoacteamide and digestion with modified trypsin for 4 h at 37 degC. After acidification, samples were filtered. ~35 micrograms of each sample was separated by direction injection and microflow LC (1 mm x 100 mm Waters ACQUITY Premier; 100 uL/min) using a gradient of 3-28% acetonrile, and MS/MS was acquired on a Thermo Exploris 480 using a 120K MS1 scan and 30K MS2 with a staggered/overlapping method. Data analysis used Spectronaut 17. Study pool QC data, and individual files used for statistical analysis, are described in the accompanying metadata.
HostingRepository	MassIVE
AnnounceDate	2024-01-31
AnnouncementXML	Submission_2024-01-31_07:46:35.212.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matt Foster
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-19 09:54:12	ID requested
⏵ 1	2024-01-31 07:46:35	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: data-independent acquisition, microflow

submitter keyword: data-independent acquisition, microflow

Contact List

Allison E. Ashley-Koch
contact affiliation	Duke University
contact email	Allison.ashleykoch@duke.edu
lab head
Matt Foster
contact affiliation	Duke University
contact email	mwfoster@duke.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000093893/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000093893/