PXD048710

PXD048710 is an original dataset announced via ProteomeXchange.

Title	Establishment and characterization of a 3D model culture system of primary human ovarian cells: Silk-Ovarioids
Description	In vitro models that mimic ovaries are instrumental in unraveling physio-pathological mechanisms underlining follicle activation and growth. Three-dimensional (3D) systems that replicate the heterogeneity and cell-cell communication among different ovarian cell types are biologically most relevant. However, such models using human primary ovarian cells have not yet been established and standardized. Here, we developed and characterized long-term cultured 3D models of primary ovarian somatic cells, isolated from normal adult tissues, using Biosilk as a scaffold. We successfully created both cortex- and medulla-derived 3D systems (Silk-Ovarioids). Through transcriptomics, proteomics, and immunostaining, we identified the presence of key ovarian somatic cell types (granulosa, stroma, endothelial and perivascular cells). Notably, the Silk-Ovarioids exhibit the formation of a proangiogenic hypoxic core, as evidenced by the formation of vessel-like structures after 6 weeks of culture. The Silk-Ovarioids have low inter/intra-batch variability and long-term culture stability, highlighting their potential as a robust step towards a bioengineered patient-specific artificial ovary.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_16:08:18.761.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akos Vegvari
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-19 04:02:42	ID requested
⏵ 1	2025-08-24 16:08:19	announced

10.1093/hropen/hoaf042;

Di Nisio V, Li T, Xiao Z, Papaikonomou K, Damdimopoulos A, V, é, gv, á, ri Á, Lebre F, Alfaro-Moreno E, Pedersen M, Svingen T, Zubarev R, Acharya G, Damdimopoulou P, Salumets A, Silk-Ovarioids: establishment and characterization of a human ovarian primary cell 3D-model system. Hum Reprod Open, 2025(3):hoaf042(2025) [pubmed]

submitter keyword: 3d model, proteomics,ovaroids

Akos Vegvari
contact affiliation	Karolinska Institutet
contact email	akos.vegvari@ki.se
lab head
Akos Vegvari
contact affiliation	Karolinska Institutet
contact email	akos.vegvari@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD048710

PRIDE project URI

• PRIDE
  1. PXD048710
     1. Label: PRIDE project
     2. Name: Establishment and characterization of a 3D model culture system of primary human ovarian cells: Silk-Ovarioids