PXD048670

PXD048670 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Enhanced Proteomic Coverage in Tissue Microenvironment by Immune Cell Subtype Library-assisted DIA-MS
Description	The objective of the protject is to develop an immune cell-enrich library-assisted DIA method that enables the comprehensive identification and quantification of immunological proteins from micro-scale samples with high sensitivity. In the project, six immune cell subtype-specific libraries including CD8+ and CD4+ T cell, B cell, NK cell, DC and MP were established, and the six subtype libraries can be consolidated into a larger library termed the immune cell-enriched library. By integrating either a single cell-type specific library or multiple libraries into the conventional library-based DIA search, the method enhances the identification coverage of the immune landscape for micro-scale colonic polyps in CRC mice. Application of the method on the MLNs of the CRC mice offers an enlarged TME landscape that reveals proteomic changes in the MLN from different tumor growth of progrssion.
HostingRepository	jPOST
AnnounceDate	2025-01-18
AnnouncementXML	Submission_2025-01-17_07:00:06.024.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jhih-Ci Yang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-18 00:16:03	ID requested
⏵ 1	2025-01-17 07:00:06	announced

Publication List

Yang JC, Hsu TH, Chen CS, Yu JH, Lin KI, Chen YJ, Enhanced Proteomic Coverage in Tissue Microenvironment by Immune Cell Subtype Library-Assisted DIA-MS. Mol Cell Proteomics, 23(7):100792(2024) [pubmed]

Yang JC, Hsu TH, Chen CS, Yu JH, Lin KI, Chen YJ, Enhanced Proteomic Coverage in Tissue Microenvironment by Immune Cell Subtype Library-Assisted DIA-MS. Mol Cell Proteomics, 23(7):100792(2024) [pubmed]

Keyword List

submitter keyword: Data-independent acquisition, Proteomics, Tumor microenvironment

submitter keyword: Data-independent acquisition, Proteomics, Tumor microenvironment

Contact List

Yu-Ju Chen
lab head
Jhih-Ci Yang
contact affiliation	Institute of Chemistry, Academia Sinica, Taiwan
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002410/