PXD048659

PXD048659 is an original dataset announced via ProteomeXchange.

Title	HIRA and dPCIF1 coordinately establish totipotent chromatin and control orderly ZGA in Drosophila embryos
Description	In order to understand the molecular mechanism underlying the action of dPCIF1 in early embryos, we carried out co-immunoprecipitation (co-IP) assays followed by mass spectroscopic analysis using dPCIF1GFP-KI early embryos (0.5–1.5 hours). In this assay, we identified a large number of dPCIF1-interacting proteins. To search for the potential factor(s) with GAF, we performed co-IP assays of early embryos, followed by mass spectroscopic analysis, and generated a dataset for GAF-interacting proteins. To identify the site(s) of dPCIF1 phosphorylation, we performed phosphor-proteomics by mass spectroscopy and identified potential phosphorylation sites.
HostingRepository	iProX
AnnounceDate	2024-01-18
AnnouncementXML	Submission_2025-06-08_19:20:17.345.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guoqiang Zhang
SpeciesList	scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-17 18:31:04	ID requested
⏵ 1	2025-06-08 19:20:18	announced

Zhang G, Miao Y, Song Y, Wang L, Li Y, Zhu Y, Zhang W, Sun Q, Chen D, embryos. Proc Natl Acad Sci U S A, 121(47):e2410261121(2024) [pubmed]

submitter keyword: drosophila，embryo, ZGA, GAF

Dahua Chen
contact affiliation	Institute of Biomedical Research, Yunnan University
contact email	chendh@ynu.edu.cn
lab head
Guoqiang Zhang
contact affiliation	Institute of Biomedical Research, Yunnan University
contact email	gqzhang@ynu.edu.cn
dataset submitter

iProX dataset URI