PXD048653

PXD048653 is an original dataset announced via ProteomeXchange.

Title	High-Throughput Identification of Calcium Regulated Proteins Across Diverse Proteomes
Description	Calcium ions play important roles in nearly every biological process, yet whole-proteome analysis of calcium effectors has been hindered by lack of high-throughput, unbiased, and quantitative methods to identify proteins-calcium engagement. To address this, we adapted protein thermostability assays in the budding yeast, human cells, and mouse mitochondria. Based on calcium-dependent thermostability, we identified 2884 putative calcium-regulated proteins across human, mouse, and yeast proteomes. These data revealed calcium engagement of novel signaling hubs and cellular processes, including metabolic enzymes and the spliceosome. Cross-species comparison of calcium-protein engagement and mutagenesis experiments identified residue-specific cation engagement, even within well-known EF-hand domains. Additionally, we found that the dienoyl-CoA reductase DECR1 binds calcium at physiologically-relevant concentrations with substrate-specific affinity, suggesting direct calcium regulation of mitochondrial fatty acid oxidation. These unbiased, proteomic analyses of calcium effectors establish a key resource to dissect cation engagement and its mechanistic effects across multiple species and diverse biological processes.
HostingRepository	PRIDE
AnnounceDate	2024-11-06
AnnouncementXML	Submission_2024-11-06_11:03:21.822.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Devin Schweppe
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-17 12:04:03	ID requested
⏵ 1	2024-11-06 11:03:22	announced

Locke TM, Fields R, Gizinski H, Otto GM, MacEwen MJS, Rusnac DV, He P, Shechner DM, McGann CD, Berg MD, Villen J, Sancak Y, Schweppe DK, High-throughput identification of calcium-regulated proteins across diverse proteomes. Cell Rep, 43(11):114879(2024) [pubmed]

10.1016/j.celrep.2024.114879;

submitter keyword: Calcium ion engagement, cell signaling, thermal stability proteomics, oxidation of polyunsaturated fatty acids

Devin Schweppe
contact affiliation	University of Washington
contact email	dkschwep@uw.edu
lab head
Devin Schweppe
contact affiliation	University of Washingt, Department of Genome Sciences
contact email	dkschwep@uw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD048653

PRIDE project URI

• PRIDE
  1. PXD048653
     1. Label: PRIDE project
     2. Name: High-Throughput Identification of Calcium Regulated Proteins Across Diverse Proteomes