PXD048644

PXD048644 is an original dataset announced via ProteomeXchange.

Title	Multidimensional proteomic landscape of intercellular signaling in pancreatic cancer
Description	We developed a multidimensional proteomic strategy to profile the glycosylated secreted and plasma membrane (S-PM) proteome of 100 human pancreatic tissue samples to an unprecedented depth, define cell-type origins of these S-PM proteins through spatial proteomics, and identify potential paracrine crosstalk, especially crosstalk mediated through tyrosine phosphorylation. Temporal dynamics during pancreatic tumor progression were further explored in a genetically engineered PDAC mouse model. Besides, generic shedding of membrane proteins in cancer and stroma regions of PDAC tumors was explored and validated through PRM-based absolute quantification.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:57:56.364.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peiwu Huang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480; Q Exactive HF-X; Orbitrap Fusion; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-17 05:38:59	ID requested
1	2024-09-10 01:41:00	announced
⏵ 2	2024-10-22 06:57:56	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Human, Spatial proteomics, Phosphoproteomics, Pancreatic cancer, shedding, PRM, Glycoproteomics, Mouse, DDA, LC-MS/MS

Ruijun Tian
contact affiliation	Department of Chemistry, Southern University of Science and Technology, Shenzhen, China
contact email	tianrj@sustech.edu.cn
lab head
Peiwu Huang
contact affiliation	Southern University of Science and Technology
contact email	11652005@mail.sustech.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD048644

PRIDE project URI

• PRIDE
  1. PXD048644
     1. Label: PRIDE project
     2. Name: Multidimensional proteomic landscape of intercellular signaling in pancreatic cancer