PXD048618

PXD048618 is an original dataset announced via ProteomeXchange.

Title	Rewiring of the phosphoproteome executes two meiotic divisions
Description	The cell cycle is ordered by a controlled network of kinases and phosphatases. To generate gametes via meiosis, two distinct and sequential chromosome segregation events occur without an intervening S phase. How canonical cell cycle controls are modified for meiosis is not well understood. Here, using highly synchronous budding yeast populations, we reveal how the global proteome and phosphoproteome change during the meiotic divisions. While protein abundance changes are limited to key cell cycle regulators, dynamic phosphorylation changes are pervasive. Our data indicate that two waves of cyclin-dependent kinase and Polo (Cdc5Polo) kinase activity drive successive meiotic divisions. These two distinct phases of phosphorylation are ensured by the meiosis-specific Spo13 protein, which rewires the phosphoproteome. Spo13 binds to Cdc5Polo to promote phosphorylation in meiosis I, particularly of substrates containing a variant of the canonical Cdc5Polo motif. Overall, our findings reveal that a master regulator of meiosis directs the activity of a kinase to change the phosphorylation landscape and elicit a developmental cascade.
HostingRepository	PRIDE
AnnounceDate	2024-03-21
AnnouncementXML	Submission_2024-03-21_09:55:36.564.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adele Marston
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-16 16:58:23	ID requested
⏵ 1	2024-03-21 09:55:36	announced

10.1038/S44318-024-00059-8;

submitter keyword: Spo13, Saccharomyces cerevisiae, kinases, phosphorylation,Meiosis

Adele Marston
contact affiliation	University of Edinburgh
contact email	adele.marston@ed.ac.uk
lab head
Adele Marston
contact affiliation	University of Edinburgh
contact email	adele.marston@ed.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048618
     1. Label: PRIDE project
     2. Name: Rewiring of the phosphoproteome executes two meiotic divisions