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PXD048603

PXD048603 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCellular Depletion of Major Cathepsin Proteases in HeLa and SH-SY5Y Cells
DescriptionProteins delivered by endocytosis or autophagy to lysosomes are degraded by exo- and endoproteases. In humans there are 15 lysosomal cathepsins (CTS) that act as important physiological regulators. The cysteine proteases CTSB and CTSL and the aspartic protease CTSD are the most abundant lysosomal proteinases and are often linked to autophagy, lysosomal storage and neurodegenerative diseases. Their concerted functions in proteolysis in the lysosome, their individual substrates, cleavage specificity, functional redundancy, and possible sequential action on substrate proteins are not well understood. To address these open points, we generated CTSB-, CTSD-, CTSL-, and CTSBDL-triple deficient (KO) human neuroblastoma-derived SH-SY5Y cells and CTSB-, CTSD-, CTSL-, CTSZ and CTSBDLZ-quadruple deficient (KO) HeLa cells. Proteome analyses of parental and CTS-depleted cells revealed an enrichment of semitryptic peptides and of lysosome/autophagy-associated proteins but also of potentially endocytosed membrane proteins like the amyloid precursor protein (APP). Amino- and carboxyterminal APP fragments accumulated in the multiple CTS-deficient cells, suggesting that APP is regularly processed by multiple CTS-mediated cleavage events.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:42:55.887.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStephan Mueller
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-16 06:49:01ID requested
12024-05-24 09:04:23announced
22024-10-22 06:42:56announced2024-10-22: Updated project metadata.
Publication List
10.1007/s00018-024-05274-4;
Gallwitz L, Bleibaum F, Voss M, Schweizer M, Spengler K, Winter D, Z, ö, phel F, M, ü, ller S, Lichtenthaler S, Damme M, Saftig P, Cellular depletion of major cathepsin proteases reveals their concerted activities for lysosomal proteolysis. Cell Mol Life Sci, 81(1):227(2024) [pubmed]
Keyword List
submitter keyword: Cathepsin, lysosome, HeLa, SH-SY5Y, proteolysis
Contact List
Stefan F. Lichtenthaler
contact affiliationDZNE Munich, Neuroproteomics, Feodor-Lynen Str. 17, 81377 Munich, Germany
contact emailstefan.lichetnthaler@dzne.de
lab head
Stephan Mueller
contact affiliationDZNE Munich Neuroproteomics
contact emailstephan.mueller@dzne.de
dataset submitter
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