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PXD048575

PXD048575 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSWATH-MS insights on butyric acid effect on mAbs production and redox homeostasis in CHO cells.
DescriptionSodium butyrate (NaBu) is well-known for its capacity to hinder cellular growth and act as a histone deacetylase inhibitor. It is commonly employed in the cultivation of recombinant Chinese hamster ovary (CHO) cell cultures to boost the production of specific proteins, such as antibodies. In this investigation, two types of CHO cell lines, namely K1 and DG44, along with their respective mAb-producing lines, K1-Pr and DG44_Pr, were cultured with or without NaBu. To analyze the proteome, a SWATH-based profiling method was utilized. The outcomes were assessed using Spectronaut 17, while STRING and Gene Ontology pathway analyses were performed using Cytoscape. The analysis confirms the known effects of NaBu on mAbs production, adding information on redox homeostasis of the cells.
HostingRepositoryPRIDE
AnnounceDate2025-05-06
AnnouncementXMLSubmission_2025-05-06_12:53:53.414.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMauro Galli
SpeciesList scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationListacetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-15 20:32:33ID requested
12025-05-06 12:53:54announced
Publication List
Galli M, Liu LC, Sim KH, Kok YJ, Wongtrakul-Kish K, Nguyen-Khuong T, Tate S, Bi X, SWATH-MS insights on sodium butyrate effect on mAbs production and redox homeostasis in CHO cells. AMB Express, 14(1):140(2024) [pubmed]
10.1186/s13568-024-01807-z;
Keyword List
submitter keyword: LC-MSMS, REDOX, SWATH-MS,CHO cells, mabs production, Butyric acid
Contact List
Xuezhi Bi
contact affiliationAnalytical Science & Technology (Protein Analytics), Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, Centros, Singapore 138668
contact emailbi_xuezhi@bti.a-star.edu.sg
lab head
Mauro Galli
contact affiliationBioprocessing Technology Institute, A-STAR, Centros, 20 Biopolis way, 138668
contact emailmauro_galli@bti.a-star.edu.sg
dataset submitter
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Dataset FTP location
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