PXD048575

PXD048575 is an original dataset announced via ProteomeXchange.

Title	SWATH-MS insights on butyric acid effect on mAbs production and redox homeostasis in CHO cells.
Description	Sodium butyrate (NaBu) is well-known for its capacity to hinder cellular growth and act as a histone deacetylase inhibitor. It is commonly employed in the cultivation of recombinant Chinese hamster ovary (CHO) cell cultures to boost the production of specific proteins, such as antibodies. In this investigation, two types of CHO cell lines, namely K1 and DG44, along with their respective mAb-producing lines, K1-Pr and DG44_Pr, were cultured with or without NaBu. To analyze the proteome, a SWATH-based profiling method was utilized. The outcomes were assessed using Spectronaut 17, while STRING and Gene Ontology pathway analyses were performed using Cytoscape. The analysis confirms the known effects of NaBu on mAbs production, adding information on redox homeostasis of the cells.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_12:53:53.414.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mauro Galli
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-15 20:32:33	ID requested
⏵ 1	2025-05-06 12:53:54	announced

Galli M, Liu LC, Sim KH, Kok YJ, Wongtrakul-Kish K, Nguyen-Khuong T, Tate S, Bi X, SWATH-MS insights on sodium butyrate effect on mAbs production and redox homeostasis in CHO cells. AMB Express, 14(1):140(2024) [pubmed]

10.1186/s13568-024-01807-z;

submitter keyword: LC-MSMS, REDOX, SWATH-MS,CHO cells, mabs production, Butyric acid

Xuezhi Bi
contact affiliation	Analytical Science & Technology (Protein Analytics), Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, Centros, Singapore 138668
contact email	bi_xuezhi@bti.a-star.edu.sg
lab head
Mauro Galli
contact affiliation	Bioprocessing Technology Institute, A-STAR, Centros, 20 Biopolis way, 138668
contact email	mauro_galli@bti.a-star.edu.sg
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048575
     1. Label: PRIDE project
     2. Name: SWATH-MS insights on butyric acid effect on mAbs production and redox homeostasis in CHO cells.