PXD048575 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SWATH-MS insights on butyric acid effect on mAbs production and redox homeostasis in CHO cells. |
Description | Sodium butyrate (NaBu) is well-known for its capacity to hinder cellular growth and act as a histone deacetylase inhibitor. It is commonly employed in the cultivation of recombinant Chinese hamster ovary (CHO) cell cultures to boost the production of specific proteins, such as antibodies. In this investigation, two types of CHO cell lines, namely K1 and DG44, along with their respective mAb-producing lines, K1-Pr and DG44_Pr, were cultured with or without NaBu. To analyze the proteome, a SWATH-based profiling method was utilized. The outcomes were assessed using Spectronaut 17, while STRING and Gene Ontology pathway analyses were performed using Cytoscape. The analysis confirms the known effects of NaBu on mAbs production, adding information on redox homeostasis of the cells. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-06 |
AnnouncementXML | Submission_2025-05-06_12:53:53.414.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mauro Galli |
SpeciesList | scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029; |
ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-15 20:32:33 | ID requested | |
⏵ 1 | 2025-05-06 12:53:54 | announced | |
Publication List
Galli M, Liu LC, Sim KH, Kok YJ, Wongtrakul-Kish K, Nguyen-Khuong T, Tate S, Bi X, SWATH-MS insights on sodium butyrate effect on mAbs production and redox homeostasis in CHO cells. AMB Express, 14(1):140(2024) [pubmed] |
10.1186/s13568-024-01807-z; |
Keyword List
submitter keyword: LC-MSMS, REDOX, SWATH-MS,CHO cells, mabs production, Butyric acid |
Contact List
Xuezhi Bi |
contact affiliation | Analytical Science & Technology (Protein Analytics), Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, Centros, Singapore 138668 |
contact email | bi_xuezhi@bti.a-star.edu.sg |
lab head | |
Mauro Galli |
contact affiliation | Bioprocessing Technology Institute, A-STAR, Centros, 20 Biopolis way, 138668 |
contact email | mauro_galli@bti.a-star.edu.sg |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048575
- Label: PRIDE project
- Name: SWATH-MS insights on butyric acid effect on mAbs production and redox homeostasis in CHO cells.