PXD048561

PXD048561 is an original dataset announced via ProteomeXchange.

Title	3. Rebalancing the proteome of pea seeds following deletion of vicilin-related genes in pea (Pisum sativum L.)
Description	Null mutations for genes encoding the major seed storage protein in pea, vicilin, were sought among a fast-neutron mutant population. Combinations of four and five mutations, where individual vicilin loci had been deleted, were generated to address the question of how removal or reduction of a major storage protein would impact on seed protein concentration and yield. While seed protein concentrations were not reduced in mutant lines, indicative of a re-balancing of the proteome, there were notable differences in their metabolite, proteomic and amino acid profiles. The genomic regions which were deleted were defined by whole genome sequencing of the parental line, JI2822 and its quintuple vicilin null derivative.
HostingRepository	PRIDE
AnnounceDate	2025-01-27
AnnouncementXML	Submission_2025-01-27_04:38:59.584.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD048561
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gerhard Saalbach
SpeciesList	scientific name: Pisum sativum (Garden pea); NCBI TaxID: 3888;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-15 06:00:22	ID requested
1	2025-01-27 04:21:43	announced
⏵ 2	2025-01-27 04:39:00	announced	2025-01-27: Updated project metadata.

10.1093/jxb/erae518;

Rayner T, Saalbach G, Vickers M, Paajanen P, Martins C, Wouters RHM, Chinoy C, Mulholland F, Bal M, Isaac P, Novak P, Macas J, Ellis N, Steuernagel B, Domoney C, Rebalancing the seed proteome following deletion of vicilin-related genes in pea (Pisum sativum L.). J Exp Bot, ():(2024) [pubmed]

10.6019/PXD048561;

submitter keyword: pea seed, mutagenesis, genetic deletion,amino acid profile, vicilin

Claire Domoney
contact affiliation	Biochemistry and Metabolism Department John Innes Centre Norwich UK
contact email	claire.domoney@jic.ac.uk
lab head
Gerhard Saalbach
contact affiliation	Biological Chemistry
contact email	gerhard.saalbach@jic.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD048561

PRIDE project URI

• PRIDE
  1. PXD048561
     1. Label: PRIDE project
     2. Name: 3. Rebalancing the proteome of pea seeds following deletion of vicilin-related genes in pea (Pisum sativum L.)