PXD048536

PXD048536 is an original dataset announced via ProteomeXchange.

Title	The kinesin motor Kif9 regulates centriolar satellite positioning during interphase
Description	Centrosomes are the principal microtubule-organizing centers of the cell, a cellular hub for protein degradation and play an essential role in mitotic spindle function that ultimately regulates chromosome segregation during mitosis. Centrosome maturation is achieved by strict control of protein acquisition and phosphorylation prior to mitosis. Defects in this process during interphase promote fragmentation of pericentriolar material once cells enter mitosis due to the increased forces exerted over the centrosome by the mitotic spindle, finally culminating in multipolar spindles and chromosome missegregation. Centriolar satellites, membrane-less assemblies of proteins involved in the trafficking of proteins toward and away from the centrosome, are thought to contribute to centrosome biogenesis. Moreover, centriolar satellites also regulate the quantity of proteolytic factors reaching the centrosome. Here we show that the microtubule plus-end directed kinesin motor Kif9 localizes to centriolar satellites and regulates their pericentrosomal localization during interphase. Lack of Kif9 leads to aggregation of satellites closer to the centrosome and increased centrosomal protein degradation that disrupts centrosome maturation and results in chromosome congression and segregation defects during mitosis. Our data show that the kinesin Kif9 controls the position of centriolar satellites relative to the centrosome and reveal roles for Kif9 and centriolar satellites in the regulation of cellular proteostasis and mitosis.
HostingRepository	PRIDE
AnnounceDate	2025-09-30
AnnouncementXML	Submission_2025-09-30_14:28:32.775.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alex Zelter
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-14 15:59:51	ID requested
⏵ 1	2025-09-30 14:28:33	announced

Vicente JJ, Wagenbach M, Decarreau J, Zelter A, MacCoss MJ, Davis TN, Wordeman L, The kinesin motor Kif9 regulates centriolar satellite positioning during interphase. Curr Biol, ():(2025) [pubmed]

10.1016/j.cub.2025.08.064;

submitter keyword: centrosome, chromosome segregation, mitosis, Kif9, microtubule-organizing center,LC-MSMS, GFP

Linda Wordeman
contact affiliation	Department of Physiology & Biophysics, University of Washington, Seattle, WA, USA
contact email	worde@uw.edu
lab head
Alex Zelter
contact affiliation	Department of Biochemistry, University of Washington, Seattle
contact email	azelter@uw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048536
     1. Label: PRIDE project
     2. Name: The kinesin motor Kif9 regulates centriolar satellite positioning during interphase