PXD048514

PXD048514 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Untargeted proteomics on Enterocloster bolteae DSM 15670 Treated with urolithin C
Description	Urolithin A is a polyphenol derived from the multi-step metabolism of dietary ellagitannins by the human gut microbiota which can affect host health. Most, but not all, individuals harbor a microbiota capable of urolithin A production; however, the enzymes that dehydroxylate its dietary precursor, urolithin C, are unknown. Here, we used a combination of transcriptomics and proteomics to reveal a urolithin C dehydroxylase (ucd) operon that dehydroxylates 9-hydroxy urolithin compounds in Enterocloster spp. Using comparative genomics, we identified Lachnoclostridium pacaense as a novel urolithin C metabolizer. Biochemical characterization and structure predictions of proteins in the Ucd complex demonstrated that dehydroxylation was both NADH- and molybdopterin-dependent and used urolithin C as a terminal electron acceptor. A meta-analysis publicly available metagenomic data revealed that both bacteria and ucd operon genes are widely distributed in gut metagenomes and likely comprise keystone species in the metabolism of urolithins by the human gut microbiota.
HostingRepository	PRIDE
AnnounceDate	2024-12-16
AnnouncementXML	Submission_2024-12-16_13:54:03.247.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD048514
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Reilly Pidgeon
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Enterocloster bolteae ATCC BAA-613; NCBI TaxID: 411902;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-12 14:13:00	ID requested
⏵ 1	2024-12-16 13:54:03	announced

Publication List

10.6019/PXD048514;
10.1101/2024.02.08.579493;

10.6019/PXD048514;

10.1101/2024.02.08.579493;

Keyword List

submitter keyword: metabolism, proteomics,Bacteria, Enterocloster bolteae, DSM 15670, urolithin A, molybdopterin, urolithin C, untargeted, oxidoreductase, urolithin

submitter keyword: metabolism, proteomics,Bacteria, Enterocloster bolteae, DSM 15670, urolithin A, molybdopterin, urolithin C, untargeted, oxidoreductase, urolithin

Contact List

Bastien Castagner
contact affiliation	Department of Pharmacology & Therapeutics, McGill University, 3655 Prom. Sir-William-Osler, Montreal, Quebec, H3G 1Y6, Canada.
contact email	bastien.castagner@mcgill.ca
lab head
Reilly Pidgeon
contact affiliation	Department of Pharmacology & Therapeutics, McGill University
contact email	reilly.pidgeon@mail.mcgill.ca
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD048514

PRIDE project URI

Repository Record List

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