PXD048507

PXD048507 is an original dataset announced via ProteomeXchange.

Title	Galectin-3 Mediated Endocytosis of the Orphan G-Protein-Coupled Receptor GPRC5A
Description	Galectins, a family of glycan-binding proteins, play crucial roles in various cellular functions, acting both at intracellular and extracellular levels. Among them, Galectin-3 (Gal-3) stands out as a unique member, belonging to the chimera subfamily. Gal-3 recruitment of plasma membrane glycosylated cargo leads to clustering and membrane bending, ultimately resulting in the formation of endocytic invaginations. An interactomic analysis on endogenous Gal-3 identified the orphan G-protein coupled receptor GPRC5A as a new binding partner for Gal-3. GPRC5A is also termed RAI3 as its expression is induced by retinoic-acid. Our results also show that GPRC5A is internalized by addition of extracellular Gal-3 and that GPRC5A trafficking pathway goes via the early and the recycling endosomes. In SW480 colorectal cancer cells, the glycosylated forms of GPRC5A interact with Gal-3 and their levels were increased by all-trans retinoic-acid (ATRA) addition. In SW480 cells, endocytic internalization of endogenous GPRC5A was achieved by extracellular Gal-3 addition but not by ATRA. This study reveals insights into the endocytic mechanisms of GPRC5A for which no specific ligand is described. Further research in this direction may unveil additional Gal-3 mediated functions in GPRC5A cellular signaling and contribute to the development of innovative therapeutic approaches.
HostingRepository	PRIDE
AnnounceDate	2025-10-09
AnnouncementXML	Submission_2025-10-09_09:05:47.079.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD048507
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Johana Chicher
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-12 06:08:11	ID requested
⏵ 1	2025-10-09 09:05:47	announced

10.6019/PXD048507;

10.3390/CELLS14191571;

submitter keyword: glycosylation,Galectin

GPRC5A

endocytosis, colorectal cancer

Sylvie Friant
contact affiliation	Université de Strasbourg, CNRS, Génétique Moléculaire, Génomique, Microbiologie (GMGM), UMR7156, 67000 Strasbourg, France
contact email	s.friant@unistra.fr
lab head
Johana Chicher
contact affiliation	Université de Strasbourg, Plateforme Protéomique Strasbourg-Esplanade, Centre National de la Recherche Scientifique
contact email	j.chicher@ibmc-cnrs.unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048507
     1. Label: PRIDE project
     2. Name: Galectin-3 Mediated Endocytosis of the Orphan G-Protein-Coupled Receptor GPRC5A