PXD048505

PXD048505 is an original dataset announced via ProteomeXchange.

Title	Mannose is crucial for mesoderm specification and symmetry breaking in gastruloids
Description	Patterning and growth are fundamental features of embryonic development that must be tightly coordinated during morphogenesis. While metabolism is known to control cell growth, how it impacts patterning and links to morphogenesis is poorly understood. To understand how metabolism impacts early mesoderm specification during gastrulation, we used in vitro mouse embryonic stem (ES) cell-derived gastruloids, due to ease of metabolic manipulations and high-throughput nature. Gastruloids showed mosaic expression of glucose transporters co-expressing with the mesodermal marker T/Bra. To understand the significance of cellular glucose uptake in development, we used the glucose metabolism inhibitor 2-deoxy-D-glucose (2-DG). 2-DG blocked the expression of T/Bra and abolishes axial elongation in gastruloids. Surprisingly, removing glucose completely from the medium did not phenocopy 2-DG treatment despite a significant decline in glycolytic intermediates occurring under both conditions. As 2-DG can also act as a competitive inhibitor of mannose in protein glycosylation, we added mannose together with 2-DG and found that it could rescue the mesoderm specification. We corroborated these results in vivomouse embryos where supplementing mannose rescued the 2-DG mediated phenotype of mesoderm specification and proximo-distal elongation of the primitive streak. We further showed that blocking production and intracellular recycling of mannose abrogated mesoderm specification. At molecular level, proteomics analysis revealed that mannose reversed glycosylation of the Wnt pathway regulator, Secreted Frizzled Receptor, Frzb, expressed in the primitive streak of the mouse embryo. Our study showed how mannose linked metabolism to glycosylation of a developmental pathway component, crucial in patterning of mesoderm and morphogenesis of gastruloids.
HostingRepository	MassIVE
AnnounceDate	2024-03-12
AnnouncementXML	Submission_2024-03-12_01:37:44.047.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adam Dowle
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Oxidation; Acetyl
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-12 05:46:53	ID requested
⏵ 1	2024-03-12 01:37:44	announced

Chaitanya Dingare, Jenny Yang, Ben Steventon. Mannose is crucial for mesoderm specification and symmetry breaking in gastruloids. https://www.biorxiv.org/content/10.1101/2023.06.05.543730v2.full.

submitter keyword: Gastruloids, Mesoderm, Mannose, N-glycosylation, Glycoprotein, Wnt

Benjamin Steventon
contact affiliation	University of Cambridge
contact email	bjs57@cam.ac.uk
lab head
Adam Dowle
contact affiliation	University of York
contact email	adam.dowle@york.ac.uk
dataset submitter

MassIVE dataset URI

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