PXD048433

PXD048433 is an original dataset announced via ProteomeXchange.

Title	IP proteomics of Arabidopsis ERF34
Description	Myc-ERF34 was expressed and precipitated using the Co-Immunoprecipitation in HEK293T Cells method. Seven-day-old Col4 seedlings were incubated in liquid 1/2 MS medium supplemented with 100 μM ABA for 3 hours. Subsequently, they were lysed using NEBT lysis buffer [20mM HEPES pH 7.5, 40mM KCl, 1mM EDTA, 1% TritonX-100] containing 1× EDTA-free Protease Inhibitor Cocktail Tablets. The resulting lysate supernatant was incubated with the precipitated Myc-ERF34. Beads were washed three times with NEBT wash buffer [20mM HEPES pH 7.5, 40mM KCl, 1mM EDTA, 0.01% TritonX-100], and then 30 µL of 4× loading buffer was added, followed by incubation at 95°C for 10 minutes
HostingRepository	iProX
AnnounceDate	2024-01-10
AnnouncementXML	Submission_2024-01-10_22:09:24.695.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Li Zhang
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-10 22:09:06	ID requested
⏵ 1	2024-01-10 22:09:25	announced

Dataset with its publication pending

submitter keyword: ERF34, Arabidopsis, interaction protein, HEK293T

Zecheng Zuo
contact affiliation	Jilin University
contact email	zuozhecheng@jlu.edu.cn
lab head
Li Zhang
contact affiliation	Jilin University
contact email	Zhang_li18@mails.jlu.edu.cn
dataset submitter

iProX dataset URI