PXD048386

PXD048386 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of a Toxoplasma gondii mutant of the ABCB7 mitochondrial transporter
Description	Toxoplasma gondii is a parasitic protist that is the agent of toxoplasmosis. It is capable of infecting a wide variety of vertebrates, including humans. The infection is mainly asymptomatic in immunocompetent patients, but in case of immunosuppression or for the congenital form of toxoplasmosis it can lead to severe pathologies with a possible fatal outcome. Like for other eukaryotes, many key cellular functions in T. gondii involve proteins containing an iron-sulfur cluster as a cofactor. Cytosolic and nuclear iron-sulfur proteins depend on a specific pathway for assembling their iron-sulfur cofactor. It was demonstrated in other eukaryotes (ie in the budding yeast model) that a sulfur-containing precursor originating from the mitochondrion and transported through the ABCB7 transporter is essential for building cytosolic iron-sulfur clusters. We have investigated the T. gondii homolog of the ABCB7 transporter by generating a specific mutant on which we performed a quantitative proteomic analysis to get insights into its involvement in the biogenesis of cytosolic and nuclear iron-sulfur proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:53:28.370.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	valerie Rofidal
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-10 07:31:34	ID requested
1	2024-08-09 02:11:39	announced
⏵ 2	2024-10-22 06:53:28	announced	2024-10-22: Updated project metadata.

10.1128/MBIO.00872-24;

submitter keyword: Toxoplasma, iron-sulfur cluster, mitochondrial transporter, CIA pathway

Sébastien Besteiro
contact affiliation	LPHI, Univ Montpellier, CNRS, INSERM, Montpellier, France
contact email	sebastien.besteiro@inserm.fr
lab head
valerie Rofidal
contact affiliation	INRAE
contact email	valerie.rofidal@inrae.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048386
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of a Toxoplasma gondii mutant of the ABCB7 mitochondrial transporter