PXD048383

PXD048383 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Bone proteomics methods optimisation for forensic investigations
Description	The application of human bone in forensic proteomics is an expanding novel purpose in the aim of successfully quantifying biological estimations used in medico-legal investigations with greater accuracy. In this project, different extraction protocols were tested on n=30 human bone samples, including S-Trap technology with two different lysis buffer, and ZipTips. Additionally, a comparison was made between data-dependent acquisition (DDA) and data-independent acquisition (DIA) mode. Results showed less missing data using S-Traps instead of the more routine reverse-phase media tips (ZipTip). The type of lysis buffer when using S-Traps does not impact largely the analysis conducted in forensic proteomic workflows. Lastly, it was found that when open-source software is used for data processing for both DDA and DIA modes, DIA has the upper advantage in terms of acquiring a larger number of proteins due to its greater sensitivity to those with a lower abundance.
HostingRepository	MassIVE
AnnounceDate	2024-05-24
AnnouncementXML	Submission_2024-05-24_10:13:34.446.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pawel Palmowski
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Deamidated
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-10 04:30:31	ID requested
⏵ 1	2024-05-24 10:13:35	announced

Publication List

Gent L, Chiappetta ME, Hesketh S, Palmowski P, Porter A, Bonicelli A, Schwalbe EC, Procopio N, Bone Proteomics Method Optimization for Forensic Investigations. J Proteome Res, 23(5):1844-1858(2024) [pubmed]

Gent L, Chiappetta ME, Hesketh S, Palmowski P, Porter A, Bonicelli A, Schwalbe EC, Procopio N, Bone Proteomics Method Optimization for Forensic Investigations. J Proteome Res, 23(5):1844-1858(2024) [pubmed]

Keyword List

submitter keyword: Bone proteomics, protein extraction, mass spectrometry, forensic science, acquisition mode

submitter keyword: Bone proteomics, protein extraction, mass spectrometry, forensic science, acquisition mode

Contact List

Noemi Procopio
contact affiliation	University of Central Lancashire
contact email	nprocopio@uclan.ac.uk
lab head
Pawel Palmowski
contact affiliation	Newcastle University NUPPA
contact email	pawel.palmowski@ncl.ac.uk
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093803/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093803/