PXD048382 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | An inhibitory segment within G-patch activators tunes Prp43-ATPase activity during ribosome assembly |
Description | Mechanisms by which G-patch activators tune the processive multi-tasking ATP-dependent RNA helicase Prp43 (DHX15 in humans) to productively remodel diverse RNA:protein complexes remain elusive. Here, a comparative study between a new activator, Tma23, and a characterized activator, Pxr1, uncovered segments that organize Prp43 function during ribosome assembly. In addition to the activating G-patch, we discovered an inhibitory segment within Tma23 and Pxr1, I-patch, that restrains Prp43 ATPase activity. Cryo-electron microscopy and hydrogen-deuterium exchange mass spectrometry show how I-patch binds to the catalytic RecA-like domains to allosterically inhibit Prp43 ATPase activity. Tma23 and Pxr1 contain dimerization segments that organize Prp43 into higher-order complexes. We posit that Prp43 function at discrete locations on pre-ribosomal RNA is coordinated through toggling interactions with G-patch and I-patch segments. This could guarantee measured and timely Prp43 activation, enabling precise control over multiple RNA remodelling events occurring concurrently during ribosome formation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-09 |
AnnouncementXML | Submission_2024-10-09_05:23:28.500.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Daniela Portugal |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-10 04:27:50 | ID requested | |
⏵ 1 | 2024-10-09 05:23:28 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Ribosome assembly, mass spectrometry, Prp43, RNA helicase |
Contact List
Vikram Panse |
contact affiliation | Institute of Medical Microbiology, University of Zürich |
contact email | vpanse@imm.uzh.ch |
lab head | |
Daniela Portugal |
contact affiliation | University of Zürich |
contact email | dportugal@imm.uzh.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048382
- Label: PRIDE project
- Name: An inhibitory segment within G-patch activators tunes Prp43-ATPase activity during ribosome assembly