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PXD048373

PXD048373 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDissecting inflammatory response in intervertebral disc degeneration: triggering of CD44 by IL-1beta
DescriptionObjectives/Design: Intervertebral Disc (IVD) degeneration has been associated with a chronic inflammatory response, but knowledge on the contribution of distinct IVD cells, namely CD44, to the progression of IVD degeneration remains elusive. Material: Bovine nucleus pulposus (NP) cells. Treatment: Stimulation with IL-1beta (10ng/ml). Methods: CD44 NP cells were sorted and compared by gene expression and proteomics with the negative counterpart. Dynamics of CD44 gene and protein expression was analyzed upon pro-inflammatory treatment. Results: CD44 has a multidimensional functional role in IVD metabolism, ECM synthesis and production of neuropermissive factors. CD44 widespread expression in NP has been partially associated with CD14 and CD45, resulting in the identification of distinct cell subsets. Conclusions: This study points out CD44 and CD44-based cell subsets as relevant targets in the modulation of the IVD pro-inflammatory/degenerative cascade.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:40:49.706.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHugo Osorio
SpeciesList scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-10 00:21:31ID requested
12024-05-22 01:14:11announced
22024-10-22 06:40:53announced2024-10-22: Updated project metadata.
Publication List
Ferreira JR, Caldeira J, Sousa M, Barbosa MA, Lamghari M, Almeida-Porada G, Gon, ç, alves RM, bovine nucleus pulposus cells with inflammation. Sci Rep, 14(1):9156(2024) [pubmed]
10.1038/s41598-024-59504-7;
Keyword List
submitter keyword: IL-1beta,intervertebral disc, nucleus pulposus, CD44, inflammation
Contact List
Raquel M. Gonçalves
contact affiliationi3S, Instituto de Investigação e Inovação em Saúde, University of Porto ICBAS, Institute of Biomedical Sciences Abel Salazar, University of Porto
contact emailraquelg@i3s.up.pt
lab head
Hugo Osorio
contact affiliationi3S - Institute for Research and Innovation in Health, University of Porto
contact emailhosorio@i3s.up.pt
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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