PXD048373 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dissecting inflammatory response in intervertebral disc degeneration: triggering of CD44 by IL-1beta |
Description | Objectives/Design: Intervertebral Disc (IVD) degeneration has been associated with a chronic inflammatory response, but knowledge on the contribution of distinct IVD cells, namely CD44, to the progression of IVD degeneration remains elusive. Material: Bovine nucleus pulposus (NP) cells. Treatment: Stimulation with IL-1beta (10ng/ml). Methods: CD44 NP cells were sorted and compared by gene expression and proteomics with the negative counterpart. Dynamics of CD44 gene and protein expression was analyzed upon pro-inflammatory treatment. Results: CD44 has a multidimensional functional role in IVD metabolism, ECM synthesis and production of neuropermissive factors. CD44 widespread expression in NP has been partially associated with CD14 and CD45, resulting in the identification of distinct cell subsets. Conclusions: This study points out CD44 and CD44-based cell subsets as relevant targets in the modulation of the IVD pro-inflammatory/degenerative cascade. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:40:49.706.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hugo Osorio |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-10 00:21:31 | ID requested | |
1 | 2024-05-22 01:14:11 | announced | |
⏵ 2 | 2024-10-22 06:40:53 | announced | 2024-10-22: Updated project metadata. |
Publication List
Ferreira JR, Caldeira J, Sousa M, Barbosa MA, Lamghari M, Almeida-Porada G, Gon, ç, alves RM, bovine nucleus pulposus cells with inflammation. Sci Rep, 14(1):9156(2024) [pubmed] |
10.1038/s41598-024-59504-7; |
Keyword List
submitter keyword: IL-1beta,intervertebral disc, nucleus pulposus, CD44, inflammation |
Contact List
Raquel M. Gonçalves |
contact affiliation | i3S, Instituto de Investigação e Inovação em Saúde, University of Porto ICBAS, Institute of Biomedical Sciences Abel Salazar, University of Porto |
contact email | raquelg@i3s.up.pt |
lab head | |
Hugo Osorio |
contact affiliation | i3S - Institute for Research and Innovation in Health, University of Porto |
contact email | hosorio@i3s.up.pt |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048373
- Label: PRIDE project
- Name: Dissecting inflammatory response in intervertebral disc degeneration: triggering of CD44 by IL-1beta