PXD048373

PXD048373 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Dissecting inflammatory response in intervertebral disc degeneration: triggering of CD44 by IL-1beta
Description	Objectives/Design: Intervertebral Disc (IVD) degeneration has been associated with a chronic inflammatory response, but knowledge on the contribution of distinct IVD cells, namely CD44, to the progression of IVD degeneration remains elusive. Material: Bovine nucleus pulposus (NP) cells. Treatment: Stimulation with IL-1beta (10ng/ml). Methods: CD44 NP cells were sorted and compared by gene expression and proteomics with the negative counterpart. Dynamics of CD44 gene and protein expression was analyzed upon pro-inflammatory treatment. Results: CD44 has a multidimensional functional role in IVD metabolism, ECM synthesis and production of neuropermissive factors. CD44 widespread expression in NP has been partially associated with CD14 and CD45, resulting in the identification of distinct cell subsets. Conclusions: This study points out CD44 and CD44-based cell subsets as relevant targets in the modulation of the IVD pro-inflammatory/degenerative cascade.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:40:49.706.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hugo Osorio
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-10 00:21:31	ID requested
1	2024-05-22 01:14:11	announced
⏵ 2	2024-10-22 06:40:53	announced	2024-10-22: Updated project metadata.

Publication List

Ferreira JR, Caldeira J, Sousa M, Barbosa MA, Lamghari M, Almeida-Porada G, Gon, ç, alves RM, bovine nucleus pulposus cells with inflammation. Sci Rep, 14(1):9156(2024) [pubmed]
10.1038/s41598-024-59504-7;

Ferreira JR, Caldeira J, Sousa M, Barbosa MA, Lamghari M, Almeida-Porada G, Gon, ç, alves RM, bovine nucleus pulposus cells with inflammation. Sci Rep, 14(1):9156(2024) [pubmed]

10.1038/s41598-024-59504-7;

Keyword List

submitter keyword: IL-1beta,intervertebral disc, nucleus pulposus, CD44, inflammation

submitter keyword: IL-1beta,intervertebral disc, nucleus pulposus, CD44, inflammation

Contact List

Raquel M. Gonçalves
contact affiliation	i3S, Instituto de Investigação e Inovação em Saúde, University of Porto ICBAS, Institute of Biomedical Sciences Abel Salazar, University of Porto
contact email	raquelg@i3s.up.pt
lab head
Hugo Osorio
contact affiliation	i3S - Institute for Research and Innovation in Health, University of Porto
contact email	hosorio@i3s.up.pt
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD048373

PRIDE project URI

Repository Record List

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